摘要
目的了解Ｆａｓ和Ｆａｓ配体（ＦａｓＬ）ｍＲＮＡ在兔角膜中的表达情况，探讨其在角膜移植中的作用。方法人工合成特异性寡核苷酸探针，应用原位分子杂交方法检测６只新西兰白兔双眼角膜上皮、角膜基质和角膜内皮层Ｆａｓ和ＦａｓＬ ｍＲＮＡ的表达。结果正常兔角膜上皮和内皮细胞层有 Ｆａｓ ｍＲＮＡ表达，基质中无表达；角膜上皮和内皮细胞层有强 ＦａｓＬ ｍＲＮＡ表达，且基质中亦有表达。兔角膜 ＦａｓＬ ｍＲＮＡ表达强于 Ｆａｓ ｍＲＮＡ的表达。结论角膜表达 Ｆａｓ和ＦａｓＬ可能与角膜移植存活有关。
Objective To investigate the expression distribution of Fas and Fas ligand(FasL) mRNA on rabbit corneas, and evaluate its effect on corneal allograft survival. Methods Based on the published oligonucleotide sequences of Fas and FasL, two oligonucleotide probes were synthesized. The expression distribution of Fas and FasL mRNA on the corneal epithelium, cornal stroma and corneal endothelium for six New Zealand rabbits were detected with in situ hybridization. Results Fas mRNA expression was present on the normal corneal epithelium and endothelium,and not present on the normal corneal stroma. FasL mRNA was present intensely on the normal corneal epithelium and endothelium,and also present on the corneal stroma. The capacity for corneal expression of FasL mRNA was higher than that of Fas mRNA. Conclusion Fas and FasL expression on cornea may be relative to corneal allograft survival.
出处
《眼科研究》
CSCD
2000年第4期289-291,共3页
Chinese Ophthalmic Research
