摘要
目的检测增生性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）玻璃体切割液标本内是否存在细胞凋亡，观察Ｆａｓ抗原（Ｆａｓ）和Ｆａｓ配体（Ｆａｓｌｉｇａｎｄ，ＦａｓＬ）与细胞凋亡的关系。方法１１例ＰＶＲ新鲜玻璃体切割液标本细胞离心涂片，末端标记（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓｆｅｒａｓｅ－ｍｅｄｉａｔｅｄｄｅｏｘｙｕｒｉｄｉｎｅ５－ｔｒｉｐｈｏｓｐｈａｔｅｎｉｃｋ－ｅｎｄｌａｂｅｌｉｎｇ，ＴＵＮＥＬ）染色检测凋亡细胞，以及Ｆａｓ、ＦａｓＬ和细胞类型特异抗原细胞角蛋白（ｃｙｔｏｋｅｒａｔｉｎ，ＣＫ）免疫组织化学检测，并与２例角膜移植后的供体眼正常对照标本的上述检查结果相对比。结果正常人视网膜Ｆａｓ和ＦａｓＬ弱表达。Ｆａｓ、ＦａｓＬ、ＣＫ和凋亡见于所有ＰＶＲ玻璃体切割液标本。ＴＵＮＥＬ阳性细胞占总细胞数的２０．５３％。７０．３５％、５１．５８％和８２．９７％的细胞分别高表达Ｆａｓ、ＦａｓＬ和ＣＫ。Ｆａｓ和凋亡的直线相关系数为０．９９（Ｐ＜０．００１）。结论ＰＶＲ眼玻璃体切割液标本可见Ｆａｓ、ＦａｓＬ的表达和细胞凋亡。Ｆａｓ和ＦａｓＬ的高表达可能与?
Objective To observe whether apoptosis was involved
in cells of aspiration fluid from vitrectomy for proliferative vitreoretinopathy(PVR),and whether
there was an association with expression of Fas antigen(Fas)and Fas ligand (FasL). Methods
Cytocentrifuge slides of 11 fresh vitreous specimens of PVR were prepared to be stained by
TUNEL method for detection of apoptosis and by immunohistochemical technique for detection
of Fas,FasL,and cytokeratin (CK),a celltype specific antigen.Results Fas and FasL were
expressed in normal human retina.Fas,FasL,CK,and apoptosis were found in all
preparations.TUNELpositive cells were 20.53% in total cells.70.35%,51.58%,and 82.97% of cells
highly expressed Fas,FasL,and CKrespectively.The linear correlation coefficient of Fas and
apoptosis was 0.99( P <0.001). ConclusionWTBZVitrectomy specimens of PVR showed
expression of Fas,FasL,and apoptosis.Prominent Fas and FasL expressions may be
associated with apoptosis of proliferating retinal pigment epithelial cells in the vitreous of PVR.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
1999年第2期78-80,共3页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金
关键词
玻璃体
视网膜病变
细胞凋亡
增生性
Vitreoretinopathy,proliferativePigment epithelium of eyeCell deathNerve growth
factors/analysisReceptors,nerve growth factor/analysisTumor necrosis factor/analysis
