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顺铂联合热疗对人胃癌耐药细胞增殖及耐药相关基因Survivin表达的影响 被引量：6

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摘要 目的通过体外实验观察顺铂联合热疗对人胃癌耐药细胞增殖及多药耐药基因Survivin表达的影响。方法选取人胃癌SGC7901/DDP细胞系进行体外培养。应用流式细胞术(FCM)检测对照组、化疗组、热疗组及热化疗组SGC7901/DDP细胞增殖的情况,并用FCM法半定量检测各组细胞中Survivin蛋白表达的变化;采用逆转录-聚合酶链反应(RT-PCR)法半定量检测4组SGC7901/DDP细胞中Survivin mRNA表达的变化。结果热疗及顺铂联合热疗都可以降低人胃癌耐药细胞SGC7901/DDP的增殖水平,并且顺铂联合热疗作用效果最明显。单纯热疗及顺铂联合热疗作用后,SGC7901/DDP细胞中Survivin蛋白及mRNA的表达明显下降,并且并顺铂联合热疗作用后下降最明显。结论热疗、顺铂联合热疗能够抑制人胃癌耐药细胞株SGC7901/DDP的增殖,并可诱导该细胞凋亡,抑制细胞周期诱导细胞凋亡是其抗肿瘤作用机制之一;下调Survivin蛋白及mR-NA的表达可能是热疗逆转人胃癌耐药细胞株SGC7901/DDP多药耐药性的机制之一。
作者 董倩 陈虎
机构地区 河北医科大学第四医院化疗科 河北医科大学第一医院肝胆外科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2013年第13期3029-3031,共3页 Chinese Journal of Gerontology
基金 河北省重点科技研究计划(No:20110445)
关键词 热疗 热化疗 胃癌耐药细胞 逆转耐药 Survivin
分类号 R733.61 [医药卫生—肿瘤]
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共引文献24

同被引文献62

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引证文献6

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中国老年学杂志
2013年 第13期

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