摘要
目的评价胰岛细胞ICA、GADA、IA-2A、IAA四种自身抗体在1型糖尿病患者中的诊断价值。方法 1型糖尿病患者51例,其中男性33例,女性18例,发病年龄20～40岁;2型糖尿病患者80例,其中男性44例,女性36例,平均年龄40～70岁;120例健康者血清,采用免疫印迹法检测血清ICA、GADA、IA-2A、IAA四种自身抗体。结果 1型糖尿病患者中,ICA、GA-DA、IA-2A、IAA四种自身抗体阳性率分别为:35.3%、52.9%、19.6%、15.7%,1型糖尿病患者中四种抗体阳性率均高于2型糖尿病患者和健康对照组,差异有统计学意义(P<0.05)。四种抗体之间在1型糖尿病患者中阳性率有差异,提示不同抗体出现在1型糖尿病患者的不同阶段。结论 ICA、GADA、IA-2A、IAA的测定对1型糖尿病的诊断具有一定价值,联合检测四种自身抗体可以提高对1型糖尿病诊断的灵敏度和互补性。
Objective To evaluate the clinical diagnostic value of four auto-antibodies against islet cells,including ICA,GAD,IA-2A and IAA,in patients with type 1 diabetes.Methods 51 patients with type 1 diabetes（33 males,18 females,aged from 20 to 40 years old）,80 patients with type 2 diabetes（44 males,36 females,aged from 40 to 70 years old） and 120 healthy subjects were recruited and detected for the serum levels of the four islet cells auto-antibodies,mentioned above,by using Western blot assay.Results The total positive rates of ICA,GAD,IA-2A and IAA were 35.3%,52.9%,19.6% and 15.7% respectively in patients with type 1 diabetes,which were significantly higher than those in patients with 2 diabetes and healthy subjects（P〈0.05）.And the positive rates of these four auto-antibodies in type 1 diabetes patients were with statistical difference,suggesting that different antibodies appeared at different stages in the patients with type 1 diabetes.Conclusion Auto-antibodies against islet cells might be valuable for the diagnosis of type 1 diabetes.Combined detection of these four auto-antibodies could increase the sensitivity and complementarity for the diagnosis of diabetes type 1.
出处
《国际检验医学杂志》
CAS
2012年第14期1690-1691,共2页
International Journal of Laboratory Medicine
关键词
糖尿病
1型
自身抗体
胰岛
diabetes mellitus,type 1； autoantibodies； islets of langerhans
