摘要
目的建立金黄地鼠颊黏膜鳞癌动物模型,观察其生物学特性。方法 40只采用0.05%4-硝基喹啉-1-氧化物(4NQO)涂抹颊黏膜,10只采用自来水涂抹颊黏膜。苏木精-伊红(HE)、转录因子蛋白(Foxm1)免疫组织化学观察第8、12周组织标本。有限稀释法体外传代培养颊黏膜组织标本,观察平板克隆形成率,检测单克隆细胞内细胞角蛋白(CK)和波形蛋白(Vim)表达,染色体分析确定细胞核型。结果第12周时26(84.6%)只颊黏膜病理为原位癌,HE染色、Foxm1免疫组织化学结果表明,符合鳞癌细胞基本特征,CK和Vim免疫组织化学阳性率约为96.0%,染色体为四倍体核型。结论 4NQO涂抹诱发金黄地鼠颊黏膜癌变,建立金黄地鼠颊黏膜鳞癌动物模型。
Objective To establish the golden hamster model of buccal squamous carcinoma and observe its biological character- istics. Methods 50 golden hamster were randomly divided into two group：experiment group （n=40） and control group（n=10）. Buccal mucosa of golden hamster were daubed by exposure 4 -nitroquinoline-1 -oxide （4NQO） in experiment group and tap water in control group. HE staining and immunohistochemistry were used to observe the tissue sample on the 8th and 12th week. The tissue samples of golden hamster buceal-mucosa cancer were used for the in-vitro subculture. Then flat cloning formation rate,expression of CK and Vim,and cell karyotype were detected. Results The observations of cell morphology and biology showed that the tissue of buceal squamous carcinoma were conformed to the basic characteristics of squamous carcinoma cell in 26 golden hamster（84.6 % ） after 12 weeks. The positive rate of CK and Vim were 96.0% by immunohistochernical staining. The Chromosomes were tetraploid karyotype. Conclusion We successfully established the golden hamster model of buccal squamous carcinoma by the daub method of 4NQO.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第21期2493-2495,2499,共4页
Chongqing medicine
基金
商丘市2011年科技发展计划项目(20115005)
关键词
癌
鳞状细胞
模型
动物
金黄地鼠
颊黏膜
carcinoma, squamous cell
animal models
golden hamster ~ buccal mucosa
