摘要
目的:研究弓形虫感染人包皮成纤维细胞(HFFs)的microRNAs(miRNAs)差异表达。方法:基因芯片分析弓形虫感染的HFFs的miRNAs差异表达,并用实时荧光定量RT-PCR法和Northern blot法验证miRNAs差异表达。结果:弓形虫感染HFFs的46种miRNAs表达量有1倍以上上调,37种miRNAs表达量有1倍以上下调。其中4种差异表达的miRNAs的实时荧光定量RT-PCR法和Northern blot法验证结果与基因芯片分析结果一致。结论:弓形虫感染可导致宿主细胞的miRNAs差异表达,差异表达的miRNAs可能在弓形虫和宿主细胞的相互作用中起调控作用。
Objective： To investigate microRNAs differential expression of human foreskin fibroblasts （HFFs） during Toxoplasma gondii infection. Methods： The microRNAs differential expression of HFFs during Toxoplasma gondii infection was analyzed by microarray, and further validated by quantitative realtime RT-PCR and Northern blot. Results： The result demonstrated that the level of 46 mature miRNAs upregulated more than 2-fold and 37 mature miRNAs down-regulated more than 2-fold in Toxoplasma gondii infected HFFs, and the validate result of 4 miRNAs level by quantitative realtime RT-PCR and Northern blot was consistent with the microarray data. Conclusion： Toxoplasma gondii infection modulates host miRNAs expression, and differential expressed miRNAs may play an important role on the interaction of the host- Toxoplasma gondii.
出处
《温州医学院学报》
CAS
2013年第7期430-434,共5页
Journal of Wenzhou Medical College
基金
浙江省自然科学基金资助项目(Y2100971)
