摘要
目的探讨耐多药结核分枝杆菌利福平相关基因rpoβ突变的特点。方法对临床结核病患者的痰液标本进行结核分枝杆菌的分离、培养、鉴定及药物敏感试验,对rpoβ基因进行PCR扩增及测序,分析rpoβ基因突变与利福平耐药的关系。结果 300份痰液标本中分离出结核分枝杆菌66株,药物敏感试验结果显示有21株耐利福平,耐药率为31.8%。21株结核分枝杆菌利福平耐药株中有16株发生氨基酸突变,突变率为76.2%,突变发生在密码子526、531、533上,以密码子531为主。结论 rpoβ基因突变是结核分枝杆菌利福平耐药的主要机制,但可能不是惟一机制。
Objective To investigate the features of rpoβ gene mutation associated with rifampicin multidrug-resistant to Mycobacterium tuberculosis. Methods Mycobacterium tuberculosis from clinical TB sputum was separated, cultured, identified, and drug sensitivity test was conductd. The rpoβ gene was amplified by PCR and sequencing, the relationship of rpoβ gene mutations and rifampin resistance was analyzed, Results 66 strains of Mycobacterium tuberculosis were isolated from 300 sputum specimens, 21 strains were resistant to rifampicin according to the results of drug susceptibility test, the resistance rate was 31.8%. 16 of 21 strains had the amino acid mutations, the mutation rate was 76.2%.Mutations was in codons 526,531,533, mainly codon 531. Conclusion rpoβ gene mutation is the primary mechanism of Mycobacterium tuberculosis resistant to rifampin, but may not be the only mechanism.
出处
《当代医学》
2013年第20期15-16,共2页
Contemporary Medicine
