摘要
目的了解浙江省耐多药结核分枝杆菌临床分离株rpo B、kat G、inh A启动子区基因突变特点,为建立本省快速耐多药检测方法提供参考。方法对浙江省第3次结核病耐药监测获得的MDR菌株扩增rpo B、kat G、inh A启动子区域基因测序后比对分析。结果 55株耐多药结核分枝杆菌rpo B、kat G、inh A启动子区域基因的突变率分别为94.6%、65.5%和10.9%,突变类型包括点突变和联合突变。在rpo B基因中,98.1%的突变发生在RRDR区域,最常见的点突变为S531L,占55.8%;在联合突变类型中,本文检测到新的突变类型为N568S+D516Y和较少报导的I561V+S531L。kat G基因突变中最常见的点突变为S315T,占80.6%;在295位点和327位点检测到同义突变。6株菌检测到inh A启动子区突变,主要为C15T突变,占83.3%。结论浙江省耐多药结核分枝杆菌耐药基因突变类型多态性明显,除常见突变类型外,存在一些特殊的突变类型,应进一步加强研究。
Objective To investigate the gene mutation characteristics of rpo B,kat G and inh A promoter region in the multi-drug resistant tuberculosis（ MDR-TB） isolates in Zhejiang,provide reference for building the suitable and fast method for detecting MDR-TB in Zhejiang. Methods MDR-TB strains were isolated from the third surveillance of drug resistance in tuberculosis in Zhejiang province,and the gene of rpo B,kat G and inh A promoter region was amplicated and comparatively analyzed after DNA-sequencing. Results The mutation rate of rpo B,kat G and inh A promoter region in all 55 MDR-TB strains were 94. 6%,65. 5% and 10. 9%,respectively. The models of mutation included point mutation and compound mutation.There was 98. 1 precent of the rpo B gene mutations occurring in the region of the RRDR,and the most frequency point mutaions model was S531 L,it accounted for 55. 8%. Two special mutation types were found in compound mutations,one was N568 S ＋D516Y,it＇s a new mutation model,the other was a rarely report models,that was I561 V ＋ S531 L. In kat G gene the most frequency point mutations model was S315 T,it accounted for 80. 6%. In codon 295 and 327,we detected the synonymous mutations. The C15 T mutation was the major types in inh A promoter region,it account for 83. 3% in whole 6 strains. Conclusion The gene mutation types has polymorphism of multidrug-resistant Mycobacterium tuberculosis in Zhejiang province. In addition to the common mutations type,there are some special types of mutations,and further research should be strengthened in this fields.
出处
《中国卫生检验杂志》
CAS
2015年第7期924-927,共4页
Chinese Journal of Health Laboratory Technology
关键词
结核分枝杆菌
耐多药
基因突变
Mycobacterium tuberculosis
Multidrug resistance
Gene mutation
