摘要
目的：研究淮南地区结核病患者临床分离RFP耐药株rpoB基因突变的特点.方法：从110例肺结核患者临床分离株中选取44例利福平耐药株和7例敏感株作rpoB基因的PCR扩增及DNA自动测序.结果：44株RFP耐药株,有43株发现rpoB基因突变,均为点突变,发生率为97.73%;其中531位点24株、526位点13株,两者占rpoB突变率的84.09%;516位突变3株,535位突变1株;另外还发现2株联合突变;耐药株中有1株未发现rpoB基因突变;7株RFP敏感株均未发现突变.结论：rpoB基因核心突变区发生突变是结核分支杆菌对利福平耐药的主要原因,其中531位丝氨酸和526位组氨酸是最常见的突变位点.
Objective：To elucidate the characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates in Huainan district. Methods：The rpoB genes of 44 rifampin- resistant strains and 7 susceptible strains seleceted from 110 clinically isolated Mycobacterium tuberculosis strains of pulmonary tuberculosis patients were amplify by PCR and sequenced. Results：The mutations of rpoB are detected in 43 （97.67 %） of 44 rifampin-resistant strains, which all are point mutations, about 84.09 % ropB gene had mutations in codon 531 or 526, including 24 strains in 531 and 13 strians in 526 ;while 3 mutations were occurred in 516 and 1 mutation was occurred in 535 ;in addition,2 union mutations were detected;there were no mutations in 1 rifampin-resistant strains,neither in 7 susceptible strains. Conclusions： Mutations in the hypervariable region of rpoB are the main reason of Mycobaeterium tuberculosis resistant to rifampin. 531 and 526 are the most common positions of mutations.
出处
《按摩与康复医学》
2010年第24期7-8,共2页
Chinese Manipulation and Rehabilitation Medicine
关键词
结核分枝杆菌
利福平
耐药
RPOB
突变
Mycobacterium tuberculosis Rifampin Drug-resistance RpoB Mutation
