摘要
利用枯草芽胞杆菌TR21喷施叶腋可以提高香蕉根系对枯萎病的抗性,为探索TR21诱导的抗性与酚类物质和木质素累积的关系,以香蕉枯萎病菌4号生理小种FOC009菌株处理的根系为阳性对照,以清水为阴性对照,比较不同处理的广粉1号(Musa spp.ABB cv.Guangfen No.1)和巴西蕉(Musa spp.AAA cv.Brazil)根系可溶性酚酸、细胞壁结合酚酸和木质素累积和差异。结果显示:处理后0、12、24、36、48、60、72 h,不同处理根系可溶性酚酸、细胞壁结合酚酸和木质素含量随时间延长逐渐增加,从处理后48 h开始,病原菌处理和生防菌处理的可溶性酚酸、细胞壁结合酚酸和木质素含量显著高于清水对照;接种72 h时,除广粉根系可溶性酚酸外,病原菌处理的可溶性酚酸、细胞壁结合酚酸和木质素含量显著高于TR21处理。结果表明TR21叶腋接种能够诱导感病品种香蕉根系酚类物质和木质素累积,与病原菌接种诱导的抗性反应表现类似,但低于病原菌诱导的水平。
Spraying banana leave axil with Bacillus subtilis strain TR21 could induce banana roots enhancing the resistance to Fusarium oxysporum f. sp. cubense. In order to discover the relationship between induced resistance by TR21 and accumulation of phenolic compounds and lignin, two susceptible banana cultivars, dwarf banana Musa spp. ABB cv. Guangfen No.1 and Cavendish banana Musa spp. AAA cv. Brazil, were chosen as research materials. Root inoculation with F. oxysporum f. sp.cubense race 4 strain Foc009 were handled as a positive control, seedlings treated with water as a negative control. The root total soluble phenolic acids （RTSPA）, cell wall bound phenolic acids （CWBPA）, lignin content of seedling roots were tested after treated at 0 h, 12 h, 24 h, 36 h, 48 h, 60 h and 72 h. The results showed that followed time expansion, RTSPA, CWBPA and lignin content increased, at and after 48 h, RTSPA, CWBPA and lignin contents in Foc009 and TR21 treatments were significantly higher than water control in different banana cultivars. Except for RTSPA in Guangfen No.l, the RTSPA, CWBPA and lignin contents of Foc009 treatments were significantly higher than TR21 treatments in two susceptible cultivars. It was concluded that spraying leave axil by TR21 could induced the susceptible banana cultivars accumulating phenolic compounds and lignin on roots, and this were similar with the action induced by F. oxysporum, but the level induced by TR21 were lower than pathogen.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第11期58-61,共4页
Guangdong Agricultural Sciences
基金
广东省农业厅农业科技推广专项(粤农计[2011]143号)
珠海市科技计划项目(PC20081078)
关键词
枯草芽胞杆菌
香蕉
酚类物质
木质素
诱导抗性
Bacillus subtilis
Musa spp.
phenolic compounds
lignin
induced resistance
