摘要
枯草芽胞杆菌R31在田间和温室对香蕉枯萎病表现出一定防效,为探索其防效产生的机制,利用耐受300μg/mL利福平和遗传稳定的突变株R31*测定其在巴西蕉和广粉一号粉蕉根际的定殖能力,进行盆栽灌根接种试验。结果显示,每株苗接种50 mL 1.78×108 CFU/mL的菌液,接种后60 d,标记菌株在香蕉根际土、根表、根内和球茎中的含量分别为4.14×106 CFU/g(干土)、1.09×107 CFU/g(鲜重)、4.66×104 CFU/g(鲜重)、1.23×103 CFU/g(鲜重);同样处理的粉蕉40 d后,根际土和根表的菌量分别为9.87×105 CFU/g(干土),7.9×104 CFU/g(鲜重)。表明突变株R31*在根际土和根表能成功定殖,但在根内和球茎的定殖量则较少,且根际土和根表的定殖量与接种浓度和每天最高气温有关。
Bacillus subtilis R31 showed a certain control effect on banana panama disease in field and greenhouse.In order to explore its bio-control mechanism,a mutant R31＊ which can tolerance rifampicin at a rate of 300 ug/ml was selected to test the colonization ability on two banana varieties which were Musa spp.AAA var.brazil and Musa spp.ABB var.Guangfen No.1.Per banana seedling inoculated 50 ml 1.78×108 CFU/mL R31＊,60 d after inoculation,the marked strains in banana rhizosphere soil,root surface,root and corm were 4.14×106 CFU/g（dry）,1.09×107 CFU/g（fresh weight,FW）,4.66×104 CFU/g（FW） and 1.23×103 CFU/g（FW）,respectively.Similarly,40 d after inoculation R31＊ in Guangfen No.1,the number of R31＊ on rhizosphere soil and root surface were 9.87×105 CFU/g（dry） and 7.9×104 CFU/g（FW）,respectively.It was found that R31＊ could colonize the rhizosphere soil and root surface of banana successfully,but the colonization quantity is less than rhizosphere in root and corm.And the population of R31＊ in rhizosphere soil and root surface have a relationship with inoculation concentration and daily maximum temperature.
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第24期59-61,68,共4页
Guangdong Agricultural Sciences
基金
广东省教育部产学研结合项目(2007B090400083)
珠海市科技计划项目(PC20081078
PC20082043)
广东省植物防疫检疫科研专项资金(粤财农[2009]225号)
关键词
枯草芽胞杆菌
香蕉
定殖
消长动态
Bacillus subtilis
banana
colonization
population fluctuation
