摘要
目的:探讨四妙勇安汤提取物对脂多糖诱导的巨噬细胞表达ICAM-1和MMP-9的影响,初步探讨四妙勇安汤提取物抗动脉粥样硬化的作用机制。方法:采用醇水提取,树脂分离获得四妙勇安汤的8个提取物,以脂多糖(LPS)刺激体外培养的小鼠巨噬细胞系RAW264.7细胞。实验共分10组,分别为正常对照组、模型组和8个提取物组。首先以5和50μg.mL-1的剂量浓度对8个提取物进行初步筛选,随后以5、10、25、50、100μg.mL-1的浓度进行复筛,以ELISA法检测各组上清液中ICAM-1:和MMP-9的含量。结果:在一定浓度范围内,1、3、6号提取物能够明显降低LPS刺激后RAW264.7细胞分泌ICAM-1:的作用(P<0.05),1、2、3、4、6号提取物能够显著降低MMP-9的分泌(P<0.01),并具有浓度依赖关系。结论四妙勇安汤部分提取物可抑制LPS刺激后巨噬细胞ICAM-1:和MMP-9的分泌,提示该方药可能通过抑制ICAM-1:和MMP-9表达发挥发挥抗动脉粥样硬化的药理作用。
Objective ：To study the effects of Simiao Yong＇an Decoction extract on intercellular adhesion molecule -1 （ICAM - 1 ） and matrix metallopeptidase - 9 （ MMP - 9） secretion of macrophage, providing basic experimental data for the development of resistance to unstable angina, the essence of Chinese medicine R ＆ D. Methods.The 8 extracts were obtained by using water - ethanol method. The model was made by stimulating mouse macrophage cell line RAW264. 7 cells with lipopolysaccharide （LPS）. There were ten groups in our experiment： control group, model group and eight extracts groups. First,5 and 50μg · mL- 1 concentrations of the 8 extracts were used for prescreening,followed by 5,10,25,50,100 μg · mL-1 concentrations for repeated screening, ICAM - 1 and MMP - 9 levels in the supernatant were measured by the ELISA assay. The effective components of the extract were screened out by high - throughput screening technology. Results. In a certain concentration range, the secretion of ICAM -1 caused by LPS injury can be reduced significantly by the extracts of the No. 1,3,6 and MMP-9 secretion can be de- creased dramatically by the extracts of the No. 1,2,3,4,6 and had a dose -effect relationship. Conclusion：The secretion of ICAM -1 and MMP- 9 in macrophages caused by LPS can be inhibited by part of extracts from Sirniao Yong＇an Decoction, and these effects may be related to inhibiting the activation of NF - KB signaling pathway.
出处
《辽宁中医杂志》
CAS
2013年第7期1479-1481,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家十一五科技重大专项-创新药物开发(2009ZX09103-
