摘要
目的观察添加剂三氯生急性毒性、遗传毒性以及生理毒性作用。方法以泥鳅为受试对象,观察三氯生在不同浓度和不同处理时间内对泥鳅急性毒性、遗传毒性及生理毒性作用,分别观察染毒后泥鳅死亡数,计算红细胞微核率和肝脏中谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性。结果与对照组比较,随着三氯生染毒浓度升高和染毒时间延长,红细胞微核率呈明显增高趋势,浓度为2.765 mg/L时,微核率达到最大,染毒后12、24、48、72 h微核率分别为(0.020 6±0.000 6)‰、(0.029 3±0.001 5)‰、(0.040 6±0.001 1)‰、(0.044 3±0.001 5)‰,随着时间推移不断增加。与对照组比较,泥鳅肝脏中GOT和GPT活性随染毒时间延长而逐渐降低,其敏感期出现在浓度为1.92 mg/L时。结论三氯生对泥鳅有显著的遗传毒性与生理毒性。
Objective To study genetic and physiological toicity of Irgansan DP300 in Misgurnus anguillicaudatus.Methods Misgurnus anguillicaudatus was exposed to different concentrations of Irgasan DP300 for different times.The mortality,micronucleus rate of red blood cells,and the activities of glutamic-oxaloacetec transaminase（GOT） and glutamic-pyruvic transaminase（GPT） in the liver of Misgurnus anguillicaudatus were determined.Results The micronucleus rate of red blood cells significantly increased with the increase of Irgansan DP300 concentration.The maximum micronucleus rate was the highest for the concentration of Irgasan Dp300 at 2.765 mg/L.Additionally,the micronucleus rate also increased with the prolongation of exposure time and varied from 0.0206±0.0006% to 0.0443±0.0015%.However,the activities of GOT and GPT gradually decreased with the prolongation of exposure time with a sensitive period for the concentration of Irgasan DP300 at 1.92 mg/L.Conclusion Irgasan DP300 exhibits significant genotoxicity and physiological toxicity in Misgurnus anguillicaudatus.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第7期1014-1016,共3页
Chinese Journal of Public Health
基金
河南省科技厅基础与前沿研究项目(112300413218)
关键词
三氯生
泥鳅
微核
谷草转氨酶
谷丙转氨酶
Irgasan DP300
Misgurnus anguillicaudatus
micronuclei
GOT
GPT
