摘要
目的:建立松塔药材的质量标准。方法:生药学研究,浸出物测定法,灰分测定法,水分测定法,TLC及HPLC色谱法。采用Phenomenex Gemini-NX C18110A色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-2%冰醋酸水溶液(90∶10),流速0.9 mL.min-1,柱温25℃,检测波长210 nm。结果:对12个不同来源的松塔药材浸出物、总灰分、酸不溶性灰分和水分进行了测定,同时对其活性成分异海松酸进行了TLC鉴别和HPLC定量研究。通过研究,将松塔药材质量标准定为:水分不超过12%,总灰分不超过1.3%,浸出物不低于3.9%。异海松醇含量不低于0.19%。结论:建立了松塔药材的质量控制标准。
Objective： To develop identification and assay methods of pinecone.Method： Pharmacognostic studies,extract mensuration,ash determination,moisture measuring method,and the bioactive constituents were analyzed by TLC and HPLC.In the HPLC method,isopimaric acid was separated on phenomenex Gemini-NX C18110A column（4.6 mm × 250 mm,5 μm） with methanol-2% acetic acid（90∶10） as the mobile phase with flow rate of 0.9 mL.min-1.The temperature of column was kept at 25 ℃.The detection wavelength was set at 210 nm.Result： The content of total ash,acid-insoluble ash,and moisture of 12 samples from different origins were determined.The qualitative and quantitative analysis of isopimaric acid by TLC and HPLC were preformed respectively.Quality specification of pinecone established through the experimental study： the moisture content less than 12%,total ash less than 1.3%,acid-insoluble ash less than 0.3%,extract not lower than 3.9%,isopimaric acid content not lower than 0.19%.Conclusion： The established method can be used for the quality specification of pinecone.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第13期68-71,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81260632)
云南省教育厅科学研究基金(2012Y328)
