摘要
目的:建立华山松中莽草酸的高效液相色谱测定方法,以分别考察华山松不同部位中莽草酸的含量。方法:色谱柱为Agilent Zorbax XDB-C18(4.6mm×150mm,5μm),流动相为甲醇∶1%磷酸水溶液=3∶97,流速0.8mL.min-1,检测波长214nm,柱温25℃。结果:莽草酸在0.041 5～0.581 0μg与峰面积线性关系良好(r=0.999 9),平均加样回收率为98.74%,RSD为0.74%(n=9)。华山松不同部位均含有莽草酸成分,且含量差异较大。结论:华山松中的莽草酸可以作为新的莽草酸资源加以开发利用。
Objective： To establish an analytical method to respectively investigate shikimic acid in different parts of P/nus armandi based on high performance liquid chromatographic experiments. Methods： Agilent Zorbax XDB-C18 column （4.6 mm×150 mm, 5μm） was used to develop the new analytical method while the mobile phase was methanol and 1% phosphoric acid solution （3：97）; the flow rate was 0.8 mL·min-1, and the detection wavelength was 214 nm; the column temperature was set at 25℃. Results： The linear relationship between the peak area and content of shikimic acid was observed in the range of 0.041 5-0.581 0μg （r=0.999 9） ; the average recovery was 98.74%, and RSD was 0.74% （n=9）. All parts of P. armandi contained shikimic acid, but the contents significantly varied from part to part. Conclusion： P. armandi can be a new resource of shikimic acid for exploitation and utilization.
出处
《大理学院学报（综合版）》
CAS
2010年第2期14-15,18,共3页
Journal of Dali University
基金
国家自然科学基金资助项目(30860365)
