摘要
本研究旨在建立小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定方法,并探讨它们的系统发生关系。在新疆、内蒙古从动物体表采集寄生蜱,形态学鉴定后,PCR扩增测序获得3种璃眼蜱的16SrRNA及线粒体色素氧化酶亚基Ⅰ基因(cytochrome oxidaseⅠ,COⅠ)序列后进行同源性分析。用Mega 5.0和Mrbayes 3.2软件分别构建系统进化树,亚洲璃眼蜱、小亚璃眼蜱样本16SrRNA序列与GenBank中已知相应蜱虫16SrRNA序列聚类,而残缘璃眼蜱COⅠ序列与GenBank中已知残缘璃眼蜱COⅠ序列聚类,与形态学鉴定结果一致。在传统形态学分类的基础上,结合分子生物学鉴定方法能简易、准确鉴定小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱。
This study is to establish a method for identifying Hyalornma anatolicum （H. anatolicum）, Hyalom rna asiatic- um （H. asiaticurn）and Hyalomrna detritum （H. detritum）with molecular markers and to learn the phylogenetic relationship of these ticks. Ticks were collected from domestic animals in Xinjiang and Inner Mongolia provinces and classified by morphology characters. Then the 16S rRNA and CO I of ticks were amplified by PCR and subsequently sequenced. Phylogenetic trees were constructed by Mega 5.0 and Mrhayes 3.2. On the phylogenetic trees based on the 16S rRNA, H. anatolicum and H. ai- saticum were clustered together with their respective classes. And H. detritum was clustered with their respective class and the H. anatolicum, H. asiaticum formed distinct branches on the phylogenetic trees based on the CO I . The method based on morphology that combined with molecular 16S rRNA and CO I seemed a simple, accuracy method for the identification of H. anatolicum, H. asiaticum and H. detritum.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第6期7-14,共8页
China Animal Husbandry & Veterinary Medicine
基金
国家科技支撑计划课题(2012BAK11B04)
