摘要
本实验观测了猪双后肢高速钢珠弹伤后外周血多形核粒细胞(Polymorphonuclear Leukocytes,PMNs)活性氧产生量、血浆和肺组织匀浆及支气管肺泡灌洗液(Bronchoalveolar Lavage Fluid,BALF)中脂质过氧化物(Lipid Peroxides,LPO)代谢产物丙二醛(Malonyldialdehyde,MDA)含量的变化。结果提示,外周血PMNs自发和在酵母多糖刺激下产生的活性氧均增加,分别在伤后4h、8h达峰值,然后逐渐减少;血浆MDA变化也经历了相似的过程,并与PMNs自发活性氧产生量的变化密切相关;肺组织匀浆和BALF中MDA在伤后24h时较正常的明显增加。文章对实验结果,特别是PMNs的功能与脂质过氧化损伤间的关系进行了讨论。
The amount of superoxide anions released by polymorphonuclear leukocytes (PMNs)and malonyldialdehyde (MDA) level in plasma,lung homoge-nate and bronchoalveolar lavage fluid (BALF) were determined in pigs after they were inflicted with gunshot wounds of steel-ball pellets.It was found that the amount of superoxide anions released spontaneously and after the stimulation of zymosa was increased and reached the peak in the 4th and 8th hour after injury respectively,and then returned to the preinjury level gradually.The changes of plasma MDA level was similar to those of superoxide anions released by PMNs.MDA level of lung homogenate and BALF was also significantly increased in the 24th hour postinjury.In addition,the relationship between PMNs function and lipid peroxidation in the plasma and the lungs was discussed.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1991年第5期451-454,共4页
Journal of Third Military Medical University
关键词
创伤
枪弹
血浆
过氧化脂质
wounds, gunshot
neutrophile
free radicals
lipid peroxides
jnalonyldialdehyde
