摘要
本实验采用单因素分析方法对蛋糕中碱性橙Ⅱ和碱性嫩黄O液相色谱检测的前处理方法进行优化。对有机溶剂提取法和固相萃取法两种前处理方法进行了对比,结果表明,这两种前处理方法对加标回收率影响的差异不显著。鉴于有机溶剂提取法成本较低,本文选择该方法并对其进行了条件优化,其最佳条件为:乙腈使用量为每次35mL,超声时间为40min,乙醚使用量为3.0mL。
Single factor analysis applied to the optimization for the pre-processing methods of determination of chrysoidin and auramine O by HPLC. The two methods of pre-processing （organic solvent extraction and Solid Phase Extraction） of HPLC was analysis. No significant different was analysis between the organic solvent extrac- tion and Solid Phase Extraction. We select the best pre-processing conditions for the determination concentration of HPLC. The best condition was as follow： the addition of acetonitrile was 35mL; the time of hypersound was 40min; the addition of ethylether was 3.0mL.
出处
《化学工程师》
CAS
2012年第12期23-25,共3页
Chemical Engineer
