摘要
目的调查广西凭祥地区莱姆病感染情况和基因型别。方法 2011年7月从广西凭祥地区采集蜱、啮齿动物和野鸟标本,分别采取煮沸法和Qiagen试剂盒提取蜱、啮齿动物脾脏以及鸟脾脏中莱姆病螺旋体基因组DNA;采用巢式PCR扩增莱姆病螺旋体5S～23SrRNA基因间隔区;对PCR扩增产物进行测序,并将序列结果与GenBank中莱姆病螺旋体5S～23SrRNA基因间隔区序列比对分析。结果从3份啮齿动物标本中检测到莱姆病螺旋体5S～23SrRNA基因间隔区片段,啮齿动物的感染率为5.66%(3/53)。其中一个序列与GenBank中Borrelia valaisiana基因型莱姆病螺旋体(序列号:HM100125.1,AB091455.1,AB091454.1,AB091453.1)同源性为100%;蜱和鸟标本中未检测到莱姆病螺旋体。结论广西凭祥地区啮齿动物中存在莱姆病螺旋体B.valaisiana基因型感染。
Objective To investigate the infection with Borrelia burgdorferi sensu lato and its genospecies in Pingxiang of Guangxi Zhuang Autonomous Region, China. Methods The specimens of ticks, rodents, and wild birds were collected in Pingxiang region of Guangxi Zhuang Autonomous Region in July 2011. Genomic DNA of B. burgdorferi sensu lato was extracted from the ticks and the spleens of rodents and wild birds by boiling method and Qiagen kit. The 5S-23S rRNA gene intergenic region of B. burgdorferi sensu lato was amplified by nested PCR; the PCR products were sequenced and aligned with the sequences of 5S-23S rRNA gene intergenic region of B. burgdorferi sensu lato deposited in GenBank to identify the genospecies of B. burgdorferi sensu lato. Results B. burgdorferi sensu lato was detected in three rodent specimens, with an infection rate of 5.66% （3/53） among the rodents. One of the sequences had a homology of 100% with B. valaisiana （accessions in GenBank： HM100125.1, AB091455.1, AB091454.1, AB091453.1）. B. burgdorferi sensu lato was not found in ticks and birds. Conclusion The infection with B. valaisiana in rodents is present in Pingxiang region of Guangxi Zhuang Autonomous Region.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2013年第3期244-246,共3页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金(81001266
81172730)~~
关键词
莱姆病螺旋体
基因型
蜱
鼠
鸟
Borrelia burgdorferi sensu lato
Genospecies
Tick
Rodent
Bird
