摘要
作者用猫作实验性急性颅脑损伤模型,采用放免测定法检测了颅脑损伤前及伤后不同时间脑脊液(CSF)和血浆中的免疫活性TRH(TRH-ir)的含量改变。结果发现,实验组颅脑伤前和伤后2、4、6h时CSF中的TRH-ir含量分别为:119.48±51.77、460.71±178.72、377.27±139.33、280.17±110.46pmol/L,血浆中的含量分别为:122.58±28.87、1158.89±163.18、909.69±160.55、545.38±132.80pmol/L。而对照组各相应时间CSF中的TRH-ir含量为:112.22±53.42、105.36±49.64、100.63±52.89、104.52±52.42pmol/L,血浆中的含量为:113.57±25.79、107.32±26.60、119.84±32.93、117.21±28.95pmol/L。实验组颅脑损伤后CSF和血浆中的TRH-ir含量均显著较对照组增高(P<0.001)。这提示颅脑损伤后有许多含TRH的神经元或神经纤维受损,从而致伤后CSF和血浆中的TRH-ir含量明显升高。这些结果表明TRH在颅脑损伤的病理生理过程中起一定作用。
The changes of immunoreactive TRH (TRH-ir) in cerebrospinal fluid (CSF) and plasma before and after acute traumatic head injury were determined with radioimmunoassay (RIA) on a feline model of acute experimental head trauma. The results showed that the concentrations of TRH-ir in experimental animals pre- and 2, 4, and 6h post-injury were 119.48±51.77, 460.71 ±178.72, 377.27± 139.33, and 280.17±110.46pmol/L in CSF, and 122.58±28.87,1158.89±163.18, 909.69±160.55, and 545.38±132.80pmol/L in plasma, respectively, while those in control animals measured at the corresponding time were 112.22±53.42, 105.36±49.64, 100.63±52.89, and 104.52±52.42pmol/L in CSF, and 113.57 ±25.79, 107.32±26.60, 119.84±31.53, and 117.21±28.95pmol/L in plasma, respectively. The contents of TRH-ir in CSF and plasma in the experimental group after traumatic head injury were significantly higher than those, in the control group (P< 0.001). This implies that a great number of TRH-containing neurons and neural fibers were damaged during traumatic head injury. The early elevation of TRH-ir contents in CSF and plasma may play a role in the pathophysiology of head trauma.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第3期215-217,共3页
Academic Journal of Second Military Medical University
关键词
颅脑损伤
TRH
免疫活性
traumatic head injury
immunoreactive TRH
cerebrospinal fluid
plasma
radioimmunoassay
