摘要
目的:以中医学"天人相应"的整体调控思想为指导,以"肾应冬"为切入点,从褪黑素高位调节的角度,通过动物实验探讨肾虚型痹证CD4+CD25+调节性T细胞(Treg)表达的变化。方法:采用胶原诱导的关节炎(CIA)大鼠模型,以冬至、夏至两个时间点,测定各组大鼠血清中CD4+CD25+Treg/CD4+Treg变化。结果:与正常组大鼠相比,CIA模型组、手术组及伪手术组CD4+CD25+Treg/CD4+Treg水平均明显降低,并且差异具有统计学意义(P<0.05,P<0.01)。说明痹证可导致Treg水平降低。在季节性比较中,冬季正常组、CIA模型组及伪手术组大鼠Treg水平明显低于夏季组,且差异具有统计学意义(P<0.05,P<0.01)。结论:Treg水平的改变具有自然节律性,人体冬天的免疫能力低于夏天,正与冬季阳气藏于内的中医理论相一致。
Objective： From the Chinese medicine point of view,man and nature should be in correspondence.Following its overall regulation as guidance and putting＇kidney should be winter＇as an entry point,the essay investigates the relationship between RA（a Chinese medicine category） Kidney deficiency CD4＋CD25＋ Treg and the season changes through animal experiments.Methods： Adopting collagen II-induced arthritis,CIA rat model which was the closest to the human RA clinical features,in the Winter Solstice and Summer solstice,the observation on the arthritis index of each rats group and the level change evaluation for the CD4＋ CD25＋ Treg/CD4＋ Treg.Results： Compared with normal group rat,the CD4＋CD25＋ Treg/CD4＋Treg level of the model of rheumatoid arthritis,CIA group,operation group,and the sham-operation group was significantly decreased and the difference had statistics significance（P〈0.05,P〈0.01）.It proved that RA could lead to the decrease of the treg level.In the season comparison,the treg level of the winter normal group,the model of rheumatoid arthritis,CIA group and the sham operation group was lower than the summer group which had statistics significance（P〈0.05,P〈0.01）.Conclusion： Changes in levels of Treg has a natural rhythm,human body immunity in winter than summer,which is as the same as the traditional Chinese medicine theory of Yang reservoirs in winter.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第7期2099-2103,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
北京中医药大学自主课题(No.JYB22)
北京中医药大学气化理论创新团队资助(No.JS011)~~
