摘要
目的:探讨不同妊娠期外周血调节性T细胞与雌激素水平变化对母胎免疫耐受的影响。方法:选择非妊娠妇女30例为对照组,早、中、晚孕及产后3天妇女各30例为研究组;应用流式细胞术检测外周血CD4+ CD25+ Treg及Foxp3+细胞占CD4+ T细胞的比例,用电化学免疫发光法测定其外周血E2含量。结果:外周血E2含量、CD4+ CD25+ Treg、CD25+ Foxp3+ Treg占CD4+细胞的比例分别为:非妊娠对照组(211.30±107.30)pmol/L、(10.99±2.64)%、(2.12±0.83)%;早孕组(16644.00±12131.20)pmol/L、(12.63±3.05)%、(2.99±1.22)%;中孕组(90846.60±36335.20)pmol/L、(19.48±5.11)%、(3.92±1.46)%;晚孕组(109354.1±40671.6)pmol/L、(17.44±5.36)%、(3.47±1.15)%;产后组(420.70±214.80)pmol/L、(13.86±2.61)%、(2.56±0.74)%。各妊娠组CD4+ CD25+ Treg和CD25+ Foxp3+ Treg均明显高于非妊娠对照组(t值分别为2.22,8.90,5.91和3.22,5.83,5.21;P均<0.05);中孕期达高峰;晚孕略有下降,但仍维持高水平,与中孕组比较无统计学差异(t=1.51和1.30,P>0.05);产后3天下降明显,但仍高于非妊娠对照组(t=4.24和2.13,P<0.05)。外周血E2含量从早孕到晚孕逐渐升高,产后迅速下降,但仍明显高于非妊娠对照组(t=4.78,P<0.01)。各妊娠组均明显高于非妊娠对照组(t值分别为7.42,13.66,14.69;P均<0.01);中、晚孕组明显高于早孕组(t=10.61和11.59,P<0.01),中、晚孕组比较无统计学差异(t=1.86,P>0.05)。结论:妊娠期CD4+ CD25+ Treg增殖和Foxp3+表达在诱导母胎免疫耐受和维持正常妊娠过程中发挥重要作用,而高水平E2对CD4+ CD25+ Treg增殖和Foxp3+表达起促进作用。
Objective： To explore the effects of regulatory T cells and changes of estrogen level on maternal - fetal immune tolerance during different pregnancy stages. Methods： 30 non - pregnant women were selected as control group, 90 pregnant women of early, middle and late pregnancy and 30 postpartum women at 3 days after birth were selected as study group; the proportions of CD4＋ ＋ CD25 ＋ Treg and Foxp3 ＋ Treg among CD4＋ ＋ T cells were detected by flow cytometly ; the serum estrogen content in peripheral blood was detected by electrochemical immunoluminescent method. Results： The serum estrogen content in peripheral blood, proportions of CD4＋ CD25＋ Treg, CD25＋ Foxp3 ＋Treg among CD4＋T cells were （211.30 ± 107.30） pmol/L, （ 10. 99 ±2. 64）% and （2.12 ±0. 83）% in non - pregnancy group, （16 644.00± 12 131.20） pmol/L, （12.63 ±3.05）% and （2.99 ± 1.22）% in early pregnancy group, （90 846.60 ±36 335.20） pmol/L, （ 19.48 ± 5. 11 ） % and （3.92 ± 1.46） % in middle pregnancy group, （ 109 354. 1 ± 40 671.6） pmol/L, （ 17.44 ± 5.36） % and （3.47 ± 1.15）% in late pregnancy group, （420. 70 ±214. 80） pmol/L, （ 13.86 ±2. 61 ）% and （2. 56 ±0. 74）% in postpartum women group. The proportions of CD4 ＋ CD25 ＋ Treg and CD25 ＋ Foxp3 ＋ Treg in pregnancy groups were significantly higher than those in non - pregnancy group （ t = 2. 22, 8.90, 5.91 and 3.22, 5.83, 5.21, respectively, P 〈 0. 05 ） , the proportions in middle pregnancy group were the highest, then decreased slightly in late pregnancy group, but there was no significant difference between late pregnancy group and middle pregnancy group （t = 1.51 and 1.30, P 〉 0. 05） , the proportions decreased significantly at 3 days after birth, but were significantly higher than those in non - pregnancy group （t = 4.24 and 2. 13, P 〈 0. 05 ） ; the serum estrogen content in peripheral blood increased gradually from early pregnancy to late pregnancy, then decreased significantly after birth, but were significantly higher than those in non - pregnancy group （ t = 4.78, P 〈 0. 01 ） ; the proportions in pregnancy groups were significantly higher than those in non - pregnancy group （ t = 7.42, 13.66, 14. 69; P 〈 0. 01 ） ; the proportions in middle and late pregnancy groups were significantly higher than those in early pregnancy group （t = 10. 61 and 11.59, P 〈0. 01 ）, but there was no significant difference between middle pregnancy group and late pregnancy group （t = 1.86, P 〉0. 05） .
出处
《中国妇幼保健》
CAS
北大核心
2010年第19期2644-2646,共3页
Maternal and Child Health Care of China
基金
深圳市科技局科技计划项目〔200603189〕
