期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

输血相关急性肺损伤SD大鼠血管生成素2和白介素10的变化 被引量：5

Change of angiopoietin-2 and interleukin-10 on transfusion-related acute lung injury in Sprague Dawley rat
原文传递
导出
摘要 目的分析患输血相关急性肺损伤(TRALI)的SD大鼠肺组织中血管生成素2(Ang-2)和白介素10(IL-10)基因相对表达量与血浆、肺组织Ang-2、IL-10浓度变化。方法 SD大鼠20只,随机分为TRALI组(n=10):腹腔注射脂多糖(LPS)(2 mg/kg)2 h后静脉输注人血浆(约1 mL/只);正常对照组(n=5):采用假手术处理;阳性对照组(n=5):静脉输注LPS(5 mg/kg)诱导急性肺损伤。应用实时荧光定量RT-PCR检测大鼠肺组织Ang-2、IL-10基因相对表达量,以管家基因b-actin为对照,2-(△△Ct)法计算目的基因相对表达情况;应用ELISA法检测大鼠血浆、肺组织中Ang-2和IL-10浓度。结果与正常对照组肺组织Ang-2、L-10基因相对表达量相比,TRALI组肺组织Ang-2基因相对表达量下调(0.28±0.06),IL-10基因相对表达量上调(22.40±1.19)。TRALI组血液、肺组织中Ang-2和IL-10浓度分别为(133.83±0.81)、(22.81±0.40)和(106.54±1.92)、(33.35±6.84)(mg/L),较正常对照组上升(P<0.05)。结论 TRALI SD大鼠血浆、肺组织中Ang-2、IL-10浓度明显高于正常对照SD大鼠,随TRA-LI病程发展,IL-10逐渐发挥其拮抗炎症作用,对Ang-2基因表达可能有反馈抑制作用。 Objective To study the change of gene expression in lung and concentration in plasma and lung of Ang-2 and IL-10 in rats with transfusion-related acute lung injury（TRALI）.Methods A total of 20 SD rats were randomly divided into control group（n = 5） with sham operation,positive control group（n = 5） with ALl induced by intravenous infusion of LPS（5 mg / kg）,and TRALI group（n = 10） treated by intraperitoneal injection of LPS 2 h before the transfusion of human plasma（about 1 mL）.Gene expression in Ang-2 and IL-10 was detected by real-time fluorescent qualification RT-PCR on the control of b-actin（housekeeping gene）.The method of 2-（△△Ct） was used to calculate the relative qualification of target genes.Results Compared with gene expression of Ang-2 and IL-10 of normal group,the gene expression of Ang-2 was downregulated（0.28 ± 0.06） and gene expression of IL-10 was upregulated（22.40 ± 1.19） in the lung tissue of TRALI group.The concentration of Ang-2 and IL-10 was higher in TRALI group（133.83 ± 0.81）,（22.81 ± 0.40） and（106.54 ± 1.92）,（33.35 ± 6.84）（mg / L） than that in the normal group.Conclusion The concentration of Ang-2 in the plasma and lung of rats in TRALI group was significantly higher than that in normal group.In the development course of TRALI,IL10 in plasma may inhibit the gene expression of Ang-2 in lung.
作者 李丽玮 李志强
机构地区 上海交通大学附属第六人民医院
出处 《中国输血杂志》 CAS CSCD 北大核心 2013年第5期439-442,共4页 Chinese Journal of Blood Transfusion
基金 上海市公共卫生重点学科建设项目"输血医学"(12WZXO201)
关键词 急性肺损伤 输血 SD大鼠 血管生成素2 白介素10 细胞因子 基因 lung injury transfusio n SD rats angiopoietin-2 interleukin-10 cytokines gene
分类号 R457.13 [医药卫生—治疗学] R446.112 [医药卫生—诊断学]
  • 相关文献

参考文献14

  • 1Popovsky MA. Transfusion and lung injury. Transfus Clin Bjol, 2001,8(3) :272-277.
  • 2Fiedler U , Reiss Y, Scharpfenecker M, et al. Angiopoietin-2 sensitizes endothelial cells to TNF -alpha and has a crucial role in the induction of inflammation. Nat MED,2006,12(2) :171-172.
  • 3Fremont RD, Koyama T, Calfee CS, et al, Acute lung injury in patients with traumatic injuries: utility of a panel of biomarkers for diagnosis and pathogenesis. J Trauma,2010,68 (5) :1121-1127.
  • 4Christie JD, Wurfel MM,Feng R,et al. Genome wide association identifies PPFIAI as a candidate gene for acute lung injury risk following major trauma. PLoS ONE, 7 (1): e28268.
  • 5http://www. ncbi. nlm. nih. gov/nuccore/198278528? report = genbank. http://www. ncbi. nlm. nih. gov/nuccore/148747381? report = genbank.
  • 6Livak KJ, Schmittgen TO. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta'Delta C (T))method. Methods,2001 ,25 (4) :402-408.
  • 7Kelher MR, Masuno T, Moore EE, et al. Plasma from stored packed red blood cells and MHC class I antibodies cause acute lung injury in a two-event in vivo rat model. Blood. 2009,113(9) :2079-2087.
  • 8Swanson K, Dwyre OM, Krochmal J , et al. Transfusion-related acute lung injury(TRALl) : current clinical and pathophysiologic considerations. Lung,2006, 184(3) : 177-185.
  • 9Toy P,Popovsky MA,Abraham E,et al. Transfusion-related acute lung injury-Definition and Review. Crit Care Med,2OO5 ,33(4) :721-726.
  • 10李丽玮,花紫菱,张惠箴,钟宁,丁在咸,李志强.输血相关急性肺损伤大鼠模型建立及肺组织病理研究[J].中国输血杂志,2012,25(11):1121-1124. 被引量：22

二级参考文献22

  • 1雷文章,韦靖江,沈文律,金立人.实验性坏死性胰腺炎多器官损害与内毒素血症的关系[J].中华实验外科杂志,1995,12(3):131-132. 被引量：117
  • 2李海东,游斌,刘国祥.LPS诱导急性肺损伤大鼠肺组织甲状腺转录因子-1表达变化[J].中国急救医学,2005,25(11):810-812. 被引量：3
  • 3Barrett NA, Kam PC. Transfusion-related acute lung injury: literature review. Anesthesia,2006,61 ( 8 ) :777-785.
  • 4FDA Center for Biologics Evaluation and Research. Fatalities reported to FDA following blood collection and transfusion, annual summary for fiscal year 2008 [ Z/OL]. 2012-04-18 [ 2012-05-20 ] http://www, fda. gov/Biologics Blood Vaccines/SafetyAvailabili- ty/Reporta Problem/Transfusion Donation Fatalities/ucm113649. htm.
  • 5Urahama N, Tanosaki R, Masahiro K, et al. TRALI after the infusion of marrow cells in a patient with acute lymphoblastic leukemia. Transfusion,2003,43 ( 11 ) : 1553-1557.
  • 6Kleinmann S, Caulfield T, Chan P, et al. Towards an understanding of transfusion-related acute lung injury:statement of a consensus panel. Transfusion ,2004,44 ( 12 ) : 1774-1789.
  • 7Popovsky MA, Moore SB. Diagnostic and pathogenetic considerations in transfusion-related acute lung injury. Transfusion, 1985,25 (6) :573-577.
  • 8Goldman M, Webert KE, Arnold DM, et al. Proceedings of a con-sensus conference:towards an understanding of TRALI. Transfus Med Rev,2005,19( 1 ) :2-31.
  • 9Chapman CE, Stainsby D, Jones H, et al. Ten years of hemovigilance reports of transfusion-related acute lung injury in the United Kingdom and the impact of preferential use of male donor plasma. Transfusion ,2008,49 (3) :440-452.
  • 10Kelher MR, Masuno T, Moore EE, et al. Plasma from stored packed red blood ceils and MHC class I antibodies cause acute lung injury in a two-event in vivo rat model. Blood,2009, 113 (9):2079- 2087.

共引文献21

同被引文献59

  • 1刘学民,徐军,刘青光,张梅,徐高峰,潘承恩.ICAM-1基因表达对大鼠急性重症胰腺炎肺损伤的影响[J].第四军医大学学报,2004,25(17):1555-1557. 被引量：7
  • 2Goldman M, Webert KE, Arnold DM, et al. Proceedings of a consensus conference: towards an understanding of TRAil[J] . Transfus Med Rev, 2005, 19( 1) : 2 -31.
  • 3Chapman CE, Stainsby D, Jones H, et al. Ten years of hemovigilance reports of transfusion - related acute lung injury in the United King?dom and the impact of preferential use of male donor plasma[J] . Transfusion, 2008,49(3): 440 -452.
  • 4FDA Center for Biologics Evaluation and Research. Fatalities re?ported to FDA following blood collection and transfusion, annual summary for fiscal year 2008[J].[EB/OLJ[2012 - 05 - 20] http://wwwfda. gov/BiologicsBlood.
  • 5Kelher MR, Masuno T, Moore EE, et al. Plasma from stored packed red blood cells and MHC class I antibodies cause acute lung injury in a two - event in vivo rat model[J]. Blood, 2009, 113 (9) : 2079 - 2087.
  • 6Silliman CC. The two - event model of transfusion - related acute lung injury[J]. Crit Care Med, 2006, 34(5 Suppl i , S124 -31.
  • 7Bux J, Sachs UJ. The pathogenesis of transfusion - related acute lung injury (TRALI)[J]. Br J Haematol, 2007, 136 ( 6 ) : 788 -799.
  • 8Gajic 0, Rana R, Winters JL, et al. Transfusion - related acute lung injury in the critically ill: prospective nested case - control study[J]. Am J Respir Crit Care Med, 2007, 176 (9) : 886 - 891.
  • 9Rana R, Fernandez - Perez ER, Khan SA, et al. Transfusion?related acute lung injury and pulmonary edema in critically ill pa?tients: a retrospective study[J]. Transfusion, 2006, 46 ( 9 ) : 1478 -1483.
  • 10Silliman CC, Curtis BR, Kopko PM, et al. Donor antibodies to HNA - 3a implicated in TRALI reactions prime neutrophils and cause PMN - mediated damage to human pulmonary microvascu?lar endothelial cells in a two - event in vitro model[J]. Blood, 2007, 109 ( 4) : 1752 -1755.

引证文献5

二级引证文献33

中国输血杂志
2013年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部