摘要
目的观察血管生成素2（Angpt-2）和腹膜透析（腹透）时腹膜血管新生的关系。方法5／6肾切除制作尿毒症大鼠模型，成模后在腹腔内植入腹透管，根据大鼠体质量每天经腹透管注入定量腹透液（4．25％，Dineal）。按腹透时间分为未腹透组、腹透10d组、28d组及56d组。假手术非尿毒症非透析大鼠为对照组。大网膜抗CD31免疫组化染色后作血管计数，观察腹膜血管新生。用实时定量PCR和Western印迹分别检测腹膜Angpt-2和血管内皮生长因子（VEGF）表达量变化，同时检测腹膜血管数和Angpt-2、VEGF表达量的关系。结果未腹透组、腹透10d、28d及56d组腹膜血管数显著高于对照组[（5±3）、（10±5）、（17±5）及（19±4）比（1±1）个／HP，均P〈0．051。腹透28d组腹膜血管数显著高于腹透10d组（P〈0．05），但与56d组差异无统计学意义。未腹透组或腹透各组腹膜Angpt-2和VEGF表达显著高于对照组（均P〈0．01），而Angpt-2和VEGF表达量并未随透析时间延长而增加。Angpt-2和VEGF表达量和腹膜血管数呈正相关（r=0．7756，P〈0．01；r=0．5223，P〈0．05）。结论尿毒症状态和腹透促进腹膜血管新生。腹膜Angpt-2表达增加和腹膜血管新生呈正相关。Angpt-2将可能成为治疗腹膜血管新生的另一靶点。
Objective To investigate the association between angiopoietin-2 （Angpt-2） and peritoneal angiogenesis in a uremic peritoneal dialysis （PD） rat model. Methods Uremic （subtotal nephrectomy） rats were established and divided into non-PD, 10 d-PD, 28 d-PD and 56 d-PD groups. Standard PD solution was applied in the study. Rats undergone sham operation without PD were used as control group. Vessel density of the peritoneum was detected and quantified with anti-CD31 immunohistochemical staining. Expressive levels of Angpt-2 and vascular endothelial growth factor （VEGF） were examined in the peritoneum by real-time PCR and Western blotting. Results The non-PD group was characterized by increased vessel density in the peritoneum compared with that of the control group [（5＋3）/HP vs （1＋1）/HP]. Progressive angiogenesis was found in 10 d-PD, 28 d-PD and 56 d-PD groups [（ 10＋5 ）/HP, （17＋5）/HP, （19＋ 4）/HP]. Furthermore, expressive levels of Angpt-2 and VEGF increased significantly in the non-PD group compared with the control （P〈0.01）, and such expressions were significantly higher in the PD groups as compared to non-PD group （P〈0.01）, but no difference was found among the PD groups. Both VEGF and Angpt-2 levels were positively correlated with vessel density（r=0.7756, P〈 0.01; r=0.5223, P〈0.05）. Conclusions Uremia and PD promote peritoneal angiogenesis in rats. Increased expression level of Angpt-2 in peritoneum is positively correlated with peritoneal angiogenesis. Angpt-2 may be a new therapeutic target of peritoneal angiogenesis.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2009年第6期415-419,共5页
Chinese Journal of Nephrology
基金
上海市科委课题基金（044119620,177QA14040,08dz1900501）
关键词
腹膜透析
新生血管化
病理性
血管生成素2
血管内皮生长因子
Peritoneal dialysis
Neovascularization, pathologic
Angiopoietin-2
Vascular endothelial growth factor
