摘要
目的:探讨糖尿病心肌梗死大鼠心肌TGF-β1和CTGF表达的变化以及肝细胞生长因子(HGF)对其影响。方法:6周龄Wistar大鼠90只随机分为5组:对照纽、糖尿病假手术纽、心肌梗死组、糖尿病心梗组和HGF治疗组,每组各18只。以高糖高脂饲料+腹腔注射链尿佐菌素(STZ)诱导建立2型糖尿病大鼠模型后再建立急性心肌梗死(AMI)模型。糖尿病模型大鼠成模后次日,HGF治疗组每天皮下注射HGF,分别观察4周及8周,检测各组心肌组织TGF-β1、CTGF的mRNA及蛋白表达情况。结果:对照组大鼠的TGF-β1、CTGF mRNA及蛋白水平表达微弱;糖尿病心梗组4周后TGF-β1、CTGF mRNA及蛋白水平表达明显升高(P<0.05),8周后表达进一步升高(P<0.01);给予HGF治疗4周后,可以部分降低TGF-β1、CTGFmRNA及蛋白水平的表达(P<0.05);给予HGF治疗8周后,可以明显降低TGF-β1、CTGF mRNA及蛋白水平的表达(P<0.01)。结论:糖尿病心肌梗死大鼠心肌TGF-β1、CTGF的表达明显增高,HGF治疗后能够降低TGF-β1、CTGF的表达,对糖尿病心肌梗死后心肌纤维化的发生有抑制作用。
To determine the changes in and the effects of hepatocyte growth factor （HGF） on transforming growth factor-β1 （TGF-β1） and connective tissue growth factor （CTGF） expression in rats with streptozotocin-induced diabetes mellitus （DM） and concomitant acute myocardial infarction （AMI）. Methods： Ninety Wistar rats 6 weeks of age were randomly assigned to control group （ n = 18） , group sham-operated DM （n=18）, group AMI （n= 18）, group DM-AMI （n = 18） and group HGF （n =18）, respectively. The rats were fed with sugar- and lipid-rich foods supplemented with streptozotocin （STZ） peritoneal injection for construction of type 2 DM model followed by AMI model. Group HGF received subeutaneous HGF injection on day 2 following construction of DM model. This entailed measurement of the expression of TGF-β1 and CTGF mRNA and protein at weeks 4 and 8. Results： The TGF-β1 and CTGF mRNA and protein expression was barely detectable in control group （both P 〉 0. 05） and significantly increased at week 4 （ both P 〈 0.05 ） and progressively augmented at week 8 （both P 〈0.01 ） in group DM-AMI. Treatment with HGF for 4 weeks led to partly suppressed TGF-β1 and CTGF InRNA and protein expression （ both P 〈 0. 05 ） , which was further attenuated at week 8 （both P 〈 0.01 ）. Conclusion： The augmented TGF--β1 and CTGF expression in rats with streptozotoein-indueed DM and concomitant AMI could 13e attenuated by the HGF therapy, suggesting that HGF may inhibit tile progression of post-infhrction myoeardial fibrosis in rats with DM.
出处
《广州医学院学报》
2013年第2期1-4,共4页
Academic Journal of Guangzhou Medical College
关键词
糖尿病
急性心肌梗死
转化生长因子-Β1
结缔组织生长因子
肝细胞生长因子
diabetes mellitus
acute myocardial infarction
transforming growth factor-β1
connectivetissue growth fiactor
hepatocyte growth factor
