摘要
对Alcaligeneseutrophus培养过程的研究表明,氮源的限制或缺乏可刺激细胞大量积累聚β羟基丁酸(PHB),但PHB合成期氮源的完全缺乏,会导致细胞的PHB合成速率迅速下降;氧的限制也可刺激A.eutrophus合成PHB,但胞内PHB的积累量远小于氮源控制下的情况。在细胞的不同生长期限制氮源的供应会明显影响PHB的发酵过程,当残留菌体浓度达到20g/L至30g/L时停止流加氨水,可以得到较好的发酵水平,细胞干重,PHB含量和PHB浓度可分别达到619g/L、80.5%和49.0g/L。
The results of the cultivation of Alcaligenes eutrophus showed that nitrogen limitation or exhaustion could stimulate the substantial accumulation of PHB. But the exhaustion of nitrogen source in PHB formation period would result in the rapid drop of PHB synthetic rate. Oxygen limitation could also stimulate the formation of PHB, but the content of PHB in the cell was much less than that in case of nitrogen controlled conditions. Obvious influences were observed on PHB fermentation when ammonia water feeding was stopped at different cell growth phases, and better results could be obtained when it was performed at 20g/L to 30g/L of residual biomass. Cell dry weight, PHB content and PHB concentration reached 61.9g/L, 80.5% and 49.0g/L, respectively under desired conditions.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第1期103-107,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金项目
轻工总会科技基金项目
