摘要
选育能高产聚羟基烷酸的优良菌株,采用最佳的发酵条件是实现细菌发酵法生产可降解塑料工业化应用的关键因素之一。因此对分离自活性污泥中的菌株Zoogloeasp.GY3进行了3L发酵罐的发酵条件研究。结果表明,罐的通气量、转速是影响最终发酵结果的主要因素,其最佳条件为在装液量为1800mL的条件下,通气量为1L/(L.min),20h之前的最佳搅拌速度为350r/min,20h之后的最佳搅拌速度为500r/min。流加控制发酵液糖浓度维持在2.5%,发酵结束后细胞生物量高达53.74g/L,PHAs质量浓度为45.9g/L,PHAs含量提高到85.4%。
Screening excellent strains which could produce polyhydroxyalkanoates with a high yield and adopting suitable fermentation conditions is one of the important factors in producing biodegradable plastic for industrial use. The fermentation condition of Zoogloea sp. GY3, which was isolated from activated sludge, was optimized in a 3L fermentor. The results showed that aeration rate and agitation speed were the main factors that impacted the biomass and PHAs accumulation. The best fermentation conditions were 1 800 mL medium, 1 L/（L·min） aeration rate and 350r/min agitation speed in the first 20h and 500r/min agitation speed after 20 h. Fedbatch fermentation results showed that biomass could reach 53.74 g/L, PHAs concentration was 45.9 g/L, and PHAs content was 85. 4% in which sugar was fed and maintained at the concentration of 2.5 %,
出处
《化工进展》
EI
CAS
CSCD
北大核心
2005年第9期1033-1036,共4页
Chemical Industry and Engineering Progress
关键词
可降解塑料
聚羟基烷酸
发酵
条件优化
流加培养
biodegradable plastic
polyhydroxyalkanoates
fermentation
optimization
fedbatch culture
