摘要
为研究不结球白菜根肿病抗性的遗传规律,本试验利用引进的结球白菜根肿病抗源与不结球白菜进行杂交,以双亲、F1、F2和BC14个世代联合群体为试验材料,采用苗期接种鉴定法进行抗性鉴定,并对其进行ISSR分子标记筛选。结果发现,不结球白菜根肿病抗性受1对显性基因控制。利用ISSR分子标记结合BSA法获得与不结球白菜根肿病抗性连锁的分子标记,命名为CR-873,该标记与抗性基因间的连锁遗传距离为9.72 cM。
The clubroot resistance gene from Chinese heading cabbage were introduced into non-heading Chinese cabbage （Brassica campestris ssp. chinensis Makino）, and the inheritance arid molecular markers of clubfoot resistance gene in parental lines, F1, F2 and BC1 of non-heading Chinese cabbage were studied by seedllng inoculation and ISSR. The results showed that the clubroot resistance in non-heading Chinese cabbage was controlled by a single dominant gene. ISSR molecular markers with bulk segregant analysis （BSA） found that primer-873 was linked to resistance gene, named CR- 873, and the genetic distance between the marker and the resistance gene was 9.72 cM .
出处
《江苏农业学报》
CSCD
北大核心
2013年第3期633-636,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家科技支撑计划项目[(2012BAD02B01-8)
(2013BAD01B04-11)]
关键词
不结球白菜
抗根肿病
分子标记
Brassica campestris ssp. chinensis
clubroot resistance
molecular marker
