摘要
[目的 ]探讨尿液中血吸虫循环抗原和抗体检测对日本血吸虫病的诊断价值。 [方法 ]用单克隆抗体夹心 ELISA法检测日本血吸虫病患者尿液中循环抗原 ,间接ELISA检测尿液中特异性抗体。 [结果 ]10例急性血吸虫病和 6 1例慢性血吸虫病患者尿液中循环抗原的阳性率分别为 6 0 %和 40 % ,特异性抗体的阳性率分别为 80 %和 6 1 7%。两者联合检测的总阳性率分别为 10 0 %和 71 7%。 10 0例健康对照者尿液中仅 3%出现假阳性。 [结论 ]检测尿液中日本血吸虫循环抗原和特异性抗体简便、实用 ,为一种非损伤性的血吸虫病诊断方法。
Objective] To assess the value of detecting circulating antigens and antibodies in urine as a noninvasive method for the diagnosis of schistosomiasis. [Methods] A sandwich ELISA and ELISA using McAb were applied to detect circulating schistosomal antigens and specific antibodies in the urine of patients with acute and chronic schistosomiasis. [Results] When the urine samples from 10 cases of acute schistosomiasis and 61 cases of chronic schistosomiasis were examined, the positive rates of circulating antigens and specific antibodies were 60%,40%, and 80%, 60 1%, respectively; when both detection was combined, the positive rates were 100% and 71 7% respectively, whereas a false positive rate of 3% of CAg or CAb was detected in the urine of 100 normal controls. [Conclusion] The detection of circulating antigen in urine is a practicable and noninvasive method for the diagnosis of schistosomiasis.
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
2000年第1期24-25,共2页
Chinese Journal of Parasitology and Parasitic Diseases
基金
"九 .五"国家重点基金!资助课题!(No 0 4 0 2 0 2 )
国家自然科学基金!资助课题 (No 39570 644)
上海市科委自然科学基金!资助课
关键词
日本血吸虫病
循环抗原
特异性抗体
诊断
Schistosomiasis japonica
circulating antigen
specific antibody.
