摘要
应用日本血吸虫抗３１／３２ｋＤ（１Ｄ＝０．９９２１ｕ）单克隆抗体双夹心酶联免疫吸附试验（双夹心ＥＬＩＳＡ）检测血吸虫循环抗原，用于城市急性血吸虫病经吡喹酮治疗５年后无再次感染患者的疗效考核，阳性率为１２．２０％。与快速法ＥＬＩＳＡ检测循环抗原的符合率为９６．３４％。与常用检测抗体的ＩＨＡ、ＥＬＩＳＡ法平行对照，阳性率基本一致。与改进的粪便孵化法比较，两法的符合率为８５．３６％。无疫水接触史健康人未见阳性反应。对肝病患者无交叉反应。结果显示双夹心ＥＬＩＳＡ法检测循环抗原敏感性高，特异性强，具有良好的抗血吸虫药物疗效考核价值。
Using against 31/32 kD(1D=0.9921u)McAb Sandwich-ELISA for detecting schistosoma japonicum circulatint antigens(Cag)the therapeutic effect of antischistosomal agents was assessed. In the reinfected patients treated by praziquantel for 5 years the positive rate was 12.20%.As compared with Fast-ELISA for rapid detecting of Cag,the coincidental rate was 96.34%.This method was also compared with IHA and ELISA for detecting circulating antibodies(Cab)and the positive rate was nearly identical.Compared with improved fecal hatching miracidium method,the coincidental rate was 85.36%,Using this method for detecting healthy individuals without exposure to infected water,no positive reaction was found.And no cross reaction was found in hepatitis cases.The results of this Sandwich-ELISA showed high sensitivity and specificity,and is valuahle in evaluation of therapeutic effect of antischistosomal agents.
出处
《同济医科大学学报》
CSCD
北大核心
1995年第4期282-284,共3页
Acta Universitatis Medicinae Tongji
关键词
血吸虫病
ELISA
单克隆抗体
循环抗原
antibody
monoclonal
enzyme-linked immunosorbent assay
circulating antigen
schistosomiasis
japonicum
assessing theraputic effect
