期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

乙酸对奶牛乳腺上皮细胞活力及CD36和FABP3基因表达的影响 被引量：6

在线阅读 下载PDF
导出
摘要 试验旨在研究在奶牛乳腺上皮细胞培养液中添加不同浓度的乙酸对细胞活力及CD36和FABP3mRNA相对表达量的影响。将传第三代的乳腺上皮细胞悬液接种于细胞培养板上，每孔加入含10％FBS的DMEM／F12培养液，于37℃、5％CO2培养箱培养48h。将培养48h的奶牛乳腺上皮细胞培养孔随机分为1个对照组和5个试验组。每组分别加入含0、4、6、8、10、12mmol／1乙酸的DMEM／F12培养液，并用1g／l无脂肪酸的BSA替代培养液中的FBS，其中0mmol／1为对照组．、结果表明，细胞相对增殖率随着乙酸浓度的增加呈显著的二次曲线增加（P=0．005），以4～8mmol／1乙酸处理组的细胞活力较高。随着乙酸添加浓度的增加，CD36的相对表达量呈显著的一次线性（P=0．011）和二次曲线（P=0．000）降低，以8～12mmol／1乙酸组较低；FABP3的表达量呈显著的一次线性（P=0．001）和二次曲线（P=0．010）增加，以10～12mmol／1乙酸组较高。乳腺上皮细胞活力与乙酸浓度呈显著的剂量依赖关系，低浓度的乙酸可促进奶牛乳腺上皮细胞活力，而高浓度则表现出抑制作用．不同浓度的乙酸处理组均下调了CD36mRNA相对表达量，上调了FABP3mRNA表达量。
作者 齐利枝 闫素梅 生冉 赵艳丽 金鹿
机构地区 内蒙古农业大学动物科学学院
出处 《饲料工业》 北大核心 2013年第11期49-52,共4页 Feed Industry
基金 奶业"973计划"[2011CB100800]
关键词 乙酸 乳腺上皮细胞 细胞活力 FABP3 CD36 奶牛
分类号 S823.91 [农业科学—畜牧学]
  • 相关文献

参考文献11

  • 1齐利枝,闫素梅,生冉,敖长金.奶牛乳腺中乳成分前体物对乳成分合成影响的研究进展[J].动物营养学报,2011,23(12):2077-2083. 被引量：12
  • 2孔庆洋,林叶,李庆章.乙酸钠和丁酸钠对奶牛乳腺脂肪酸合成相关基因的影响[J].中国乳品工业,2012,40(3):15-17. 被引量：8
  • 3Maxin G, Glasser F, Hurtaud C, et al. Combined effects of trans-10, cis-12 conjugated linoleic acid, propionate, and ac- etate on milk fat yield and composition in dairy cows[J]. Jour- nal of Dairy Science, 2011, 94:2051-2059.
  • 4Bionaz M, Loor J J. Gene networks driving bovine milk fat synthesis during the lactation cycle[J]. BMC Genomies,2008a, 9:366.
  • 5Bionaz M, Loor J J. ACSL1, AGPAT6, FABP3, LPINI, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation[J]. The Journal of Nutrition, 200811.138:1019-1024.
  • 6Liv A K K J, Sehmittgen T D. Analysis of relative gene ex- pression data using real-time quantitative PCR and tile 2 ^ △△α method[J]. Methods, 2001, 25(4): 402-408.
  • 7Zhou Y, Akers R M, Jiang H. Growth hormone can induce ex- pression of tbur major milk protein genes in transfected MAC- T cells[J]. Journal of Dairy Science, 2008, 91 : 100-108.
  • 8胡菡.中国荷斯坦奶牛乳腺上皮细胞体外培养体系的建立与应用[D].甘肃农业大学博士学位论文,2010.
  • 9Spitsberg V L, Matitashvili E, Gorewit R C. Assowiation and coexpression of fatty-acid-binding protein and glycoprotein CD36 in the bovine mammary gland[J]. European Journal of Biochemistry, 1995, 230: 872-878.
  • 10Yonezawa T, Yonekura S, Sanosaka M, et al. Oetanoate stim- ulates cytosolic triacylglycerol accumulation and CD36 mRNA expression but inhibils Acetyl coenzyme A carboxyl- ase activity in primary cultured bovin e mammary epithelial cells[J]. Journal of Dairy Research, 2004, 71:398-404.

二级参考文献14

  • 1王佳堃,刘建新.泌乳反刍动物乳腺的氨基酸代谢[J].中国农业科学,2005,38(7):1453-1457. 被引量：8
  • 2WELLNITZ O,KERR D E.Cryopreserved Bovine Mammary Cellsto Model Epithelial Response to Infection[J].Vet Immunol Im-munopathol,2004,101(3-4):191-202.
  • 3SAKAMOTO K,KOMATSU T,KOBAYASHI T,et al.GrowthHmrmone Acts on the Synthesis and Secretion of Alpha-casein inBovine Mammary Epithelial Cells[J].J.Dairy Res,2005,72(3):264-270.
  • 4LPEZ-LUNA P,MAIER I,HERRERA E.Carcass and Tissue FatContent in the Pregnant Rat[J].Biol.Neonate,1991,60:29-38.
  • 5GRIINARI J M,CORL B A,LACY S H,et al.Conjugated LinoleicAcid is Synthesized Endogeneously in Lactating Dairy Cows by Deta-9 Desaturase[J].J.Nure,2000,30:2285-2291.
  • 6BRISKEN C,HEINEMAN A,CHAVARRIA T,et al.EssentialFunction of Wnt-4 in Mammary Gland Development Downstream ofProgesterone Signaling[J].Genes Dev,2000,14:650-654.
  • 7SHIMANO H.Sterol Regulatory Element-binding Proteins(SREBPs):Transcriptional Regulators of Lipid Regulators of LipidSynthetic Genes[J].Progress in Lipid Research,2001,40(6):439-452.
  • 8SILLINGFORD J M,HENNIGHAUSEN L.Experimental MouseGenetics-answering Fundamental Questions about Mammary GlandBiology[J].Trends Endocrinol Metab,2001,12(9):402-408.
  • 9BAUMGARD L H,MATITASHVILI E,CORL B A,et al.Trans-10,Cis-12 Conjugated Linoleic Acid Decreases Lipogenic Rates andExpression of Genes Involved in Milk Lipid Synthesis in Dairy Cows[J].J.Dairy,2002:2155-2163.
  • 10Huhtanen P,Vanhatalo A,Varvikko T.Effects of abomasal infusionsof histidine,glucose,and leucine on milk production and plasmametabolites of dairy cows fed grass silage diets. Journal of Dairy Science . 2002

共引文献17

同被引文献72

  • 1Coleman R A, Lewin T M, Muoio D M. Physiological and nutritional regulation of enzymes of triacylglycerol synthesis[J]. Annual Review of Nutrition, 2000, 20, 77-103.
  • 2Peterson D G, Matitashvili E A, Bauman D E. The inhibitory effect of trans -10, cis-12 CLA on lipid synthesis in bovine mam- mary epithelial cells involves reduced proteolytic activation of the transcription factor SREBP-I[J]. J Nutr, 2004, 134(10): 2523- 2527.
  • 3Georgiadi A, Kersten S. mechanisms of gene regulation by fatty acids[J]. Adv Nutr, 2012, 3(2): 127-134.
  • 4Duplus E, Glorian M, Forest C. Fatty acid regulation of gene tran- scription [J]. J Biol Chem, 2000, 275(40): 30749 -30752.
  • 5Sampath H, Ntambi J M. Polyunsaturated fatty acid regulation of genes of lipid metabolism[J]. Annu Rev Nutr, 2005, 25: 317-340.
  • 6Lin X, Loot J J, Herbein J H. Trans10, cisl2-18:2 is a more po- tent inhibitor of de novo fatty acid synthesis and desaturation than cis9, transll-18:2 in the mammary gland of lactating mice[J]. J Nutr, 2004, 134(6): 1362-1368.
  • 7Chilliard Y, Ferlay A, Mansbridge R M, et al. Ruminant milk fat plasticity: Nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids[J]. Annales de Zootechnie, 2000, 49 (3): 181-205.
  • 8Coleman R A, Lee D P. Enzymes of triacylglycerol synthesis and their regulation[J]. Prog Lipid Res, 2004, 43: 134-176.
  • 9Bionaz M and Loor JJ. Gene networks driving bovine milk fat syn- thesis during the lactation cycle[J]. BMC Genomics, 2008(9): 366-387.
  • 10Anderson S M, Rudolph M C, McManaman J L, et al. Key stages in mammary gland development. Secretory activation in the mam- mary gland: it's not just about milk protein synthesis[J]. Breast Cancer Res, 2007, 9(1): 204.

引证文献6

二级引证文献15

饲料工业
2013年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部