摘要
目的观察转录因子ZEB1在乳腺癌组织中的表达及其与临床病理特征的关系。方法采用免疫组化SP法检测20例正常乳腺组织、33例乳腺增生组织、9例乳腺导管内癌组织、67例浸润性导管癌组织、16例浸润性微乳头状癌组织及20例浸润性小叶癌组织中ZEB1的表达。结果正常乳腺、乳腺增生症及乳腺导管内癌组织中ZEB1呈阴性,而浸润性导管癌、浸润性微乳头状癌、浸润性小叶癌组织中ZEB1的阳性率分别为13.43%(9/67)、37.50%(6/16)和55.00%(11/20);乳腺浸润性导管癌与浸润性小叶癌组织中ZEB1阳性率的差异有统计学意义(P<0.01),浸润性导管癌与浸润性微乳头状癌组织中ZEB1阳性率的差异有统计学意义(P<0.05)。乳腺癌组织中有淋巴结转移者ZEB1阳性率为32.20%(19/59),无淋巴结转移者阳性率为13.20%(7/53),两者相比差异有统计学意义(P<0.05)。103例浸润性乳腺癌中ZEB1的阳性率与癌细胞的Ki-67增殖指数具有相关性(P<0.05),Ki-67高增殖指数者ZEB1阳性率较高。ZEB1表达与ER、PR、HER-2的表达无关(P>0.05)。结论 ZEB1在乳腺癌恶性肿瘤中的表达显著升高。ZEB1与乳腺癌的增殖、浸润及淋巴结转移有关,检测ZEB1对研究乳腺癌侵袭转移及判断患者的预后有一定的价值。
Purpose To investigate the expression of ZEB1, an epithelial-mesenchymal transition （EMT） transcription factor, in breast carcinoma tissues and to examine the correlation between ZEB1 expression and clinicpathological features in patients with breast cancer. Methods Immunohistochemistry was used to detect the expression of ZEB1 in 20 cases of normal breast tissues, 33 cases of breast hyperplasia, 9 cases of ductal carcinoma in situ （DCIS）, 67 cases of invasive ductal carcinoma, 16 cases of invasive micropap- illary carcinoma, and 20 cases of invasive lobular carcinoma. The correlation was investigated between ZEB1 expression and age, tumor size, estrogen, progesterone and HER-2 receptor status of the patients. Results Our data demonstrated that there was no positive ex- pression of ZEB1 observed in normal breast tissues, breast hyperplasia and DCIS. While the positive rates of ZEB1 in invasive ductal carcinoma, invasive micropapillary carcinoma, and invasive lobular carcinoma were 13.43% （9/67）, 37.50% （6/16） and 55% （ 11/20）, respectively. The differences of positive rates between invasive ductal carcinoma and invasive lobular carcinoma were statis- tically significant （P 〈 0. O1 ）. The same trend was observed between invasive ductal carcinoma and invasive micropapillary carcinoma （P 〈0.05）. The positive rates in the patients with and without lymph node metastasis were 32. 20% （19/59） and 13.20% （7/53）, respectively. The difference between these two groups was statistical significance （ P 〈 0. 01 ）. The positive expression rate of ZEB1 in 103 cases of invasive carcinoma was significantly correlated with the expression of proliferation index Ki-67 （ P 〈 0. 05 ） , indicating that cases with higher proliferation index tend might have higher ZEB1 positive rate. The expression of ZEB1 had no correlation with age, tumor size, estrogen, progesterone and HER-2 receptor status of the patients （P 〉 0.05）. Conclusions The expression of ZEB1 is upregulated in breast carcinomas with more aggressive activity, and it is correlated with the proliferation, invasiveness and lymph node metastasis of breast carcinoma. Therefore, the detection of ZEB1 expression may be helpful for the investigation of invasion and metas- tasis, and judgment of prognosis in patients with breast carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2013年第6期594-597,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
乳腺肿瘤
ZEB1转录因子
肿瘤转移
免疫组织化学
breast neoplasms
ZEB1 transcription factor
cancer metastasis, immunohistochemistry
