摘要
目的研究性激素水平、雌激素受体(ER)与孕激素受体(PR)表达在乳腺增生病辨证分型中的变化及其意义。方法将研究对象分为正常组、肝郁气滞型组、痰瘀互结型组和冲任失调型组,采用酶免法和免疫组织化学法,检测各组血清雌激素(E2)、孕酮(P)、泌乳素(PRL)水平及乳腺组织ER、PR的表达。结果乳腺增生各证型组与正常组E2、PRL比较均明显升高(P<0.01)。各证型中,肝郁气滞型组PRL高于冲任失调型组(P<0.01);冲任失调型组P值与肝郁气滞型组和痰瘀互结型组比较,差异有显著性(P<0.01)。其余各项性激素指标在乳腺增生病各证型中虽有所差异,但均无统计学意义。乳腺增生组织中ER、PR表达高于正常组(P<0.01),各证型中冲任失调型组高于肝郁气滞型和痰瘀互结型组(P<0.05)。结论血清性激素水平失调以及ER、PR高表达致使乳腺组织对雌激素的敏感性增强可能是乳腺组织增生的重要因素之一;各证型中性激素水平、ER、PR表达的差异性可作为乳腺增生病辨证分型的参考指标。
Objective To explore the correlation of traditional chinese medicine（TCM） differentiation of mammary hyperplasia disease and serum sex-hormone level, the expression of estrogen and progesterone receptor. Methods The regularity of estrogen progesterone and prolactin change of all types of mammary hyperplasia disease was measured with MEIA. The expression of estrogen and progesterone receptor in the mammary tissue was measured with the S-P method. Results Serum estrogen and prolactin levels increased, progestogen lowered （P 〈 0.01 ） significantly in all type groups of mammary hyperplasia disease than normal control group. The level of prolactin in stagnation gang-qi group was higher significantly than that in stagnancy of phlegm and blood stasis group. The level of progestogen in disorder of thoroughfare vessel and conception vessel type group was higher significantly than that in stagnation gang-qi group and stagnancy of phlegm and blood stasis group （P 〈 0.01）. The expression of estrogen and progesterone receptor in all type groups of mammary hyperplasia disease was higher significantly than that in normal control group （P〈 0.01 ）, the expression of estrogen and progesterone receptor in disorder of thoroughfare vessel and conception vessel type group was higher significandy than that in stagnation gang qi group and stagnancy of phlegm and blood stasis group（P〈0.05）. Conclusion The disorder of serum sex-hormone and the high expression of estrogen and progesterone receptor in the mammary hyperplasia disease may be a clue in different TCM type.
出处
《福建医科大学学报》
2005年第3期291-294,共4页
Journal of Fujian Medical University
基金
福建省自然科学基金资助项目(C0010016)
关键词
纤维囊性病
乳腺
辨证分型
性激素类
受体
雌激素
受体
孕硐
mammary hyperplasia
syndrome differ classification
sex hormone
estrogens receptors
progesterone receptors
