摘要
用高效液相色谱法测定血浆中的阿苯达唑(Alb)及其代谢物阿苯达唑亚砜(AlbSO)和阿苯达唑砜(AlbSP)。检测血浆中的Alb和AlbSO的限度为5ng·ml^(-1),AlbSP的为10ng·ml^(-1)。应用本法检测Alb、AlbSO和AlbSP的回收率均在90％以上。
A high performance liquid chromatography method was used for quantitative determination of albendazole (Alb) and its major metabolites, albendazole sulfoxide (AlbSO) and albendazole sulphone (AlbSP) in plasma of rabbits and mice. A ODS (5μm) column ditted with a variable wavelength UV detector at 292 nm was used for assay, and mebendazole was used as internal standard. The mobile phase used was 48% methanol / H2O (v / v, containing HClO4 0.6 ml·L-1) with a flow rate of 1 ml. min-1. The detectable limitation of Alb or AlbSO in plasma was 5 ng·ml-1, while that of AlbSP was 10 ng·ml-1. The recoveries of Alb, AlbSO and AlbSP by the method were 93.5%, 96.6% and 99.2%, respectively. The results showed that the method is simple, precise and sensitive and is suitable for pharmacokinetic study and microdetermination of the relevant substances present in the tissues.
出处
《地方病通报》
1991年第3期7-11,共5页
Endemic Diseases Bulletin
关键词
高效液相色谱法
阿苯达唑
HPLC
Plasma
Albendazole
Albendazole sulfoxide
Albendazole sulphone
