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人参皂苷Rb1对阿尔茨海默病模型鼠海马区神经元NeuN表达的影响 被引量：3

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摘要 目的观察人参皂苷Rb1对阿尔茨海默病(AD)模型鼠海马区神经元NeuN表达的影响,探讨人参皂苷Rb1对AD的治疗作用。方法选用10月龄SAMP8鼠作为实验鼠,对照鼠为SAMR1鼠,等量分至给药组(SAMP8+GSRb1组和SAMR1+GSRb1组)和未给药组(SAMP8组和SAMR1组)。给药组腹腔注射2.5 mg/kg人参皂苷Rb1 1次/d,连用4周,未给药组腹腔注射同等剂量生理盐水。第1周后所有鼠腹腔注射50 mg/kg Brdu 2次/d,连续3周注射后,10%水合氯醛麻醉后灌注取脑,脑组织固定脱水,用冰冻切片机切片后孵育一抗二抗,激光共聚焦扫描显微镜扫描,采用免疫荧光和免疫荧光双标方法观察各组海马区齿状回Brdu/NeuN表达情况。结果与SAMR1+GSRb1组(0.25±0.10)比较,SAMP8+GSRb1组(0.50±0.10)Brdu/NeuN表达明显增多,差异有统计学意义(t=3.95,P=0.005 2);与SAMR1组(0.54±0.08)比较,SAMP8组(0.40±0.08)Brdu/NeuN表达减少,差异有统计学意义(t=4.06,P=0.003 6)。与SAMP8组比较,SAMP8+GSRb1组Brdu/NeuN表达明显增多,差异有统计学意义(t=2.82,P=0.022 4)。与SAMR1组比较,SAMR1+GSRb1组Brdu/NeuN表达增多,差异有统计学意义(t=6.97,P=0.0001)。结论人参皂苷Rb1可以增加AD模型鼠海马齿状回NeuN表达,证明人参皂苷Rb1可促进神经元NeuN再生,增强神经元抗损伤与凋亡的能力,为AD治疗提供可靠依据。
作者 于小琪 向君
机构地区 广东省广州市第一人民医院老年病科
出处 《广东医学》 CAS CSCD 北大核心 2013年第9期1335-1336,共2页 Guangdong Medical Journal
关键词 阿尔茨海默病 人参皂苷RBL SAM鼠 海马区齿状回 NEUN
分类号 R286.0 [医药卫生—中药学]
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参考文献12

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广东医学
2013年 第9期

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