摘要
目的探讨灯台叶醇提物抗肿瘤作用及其机制。方法以移植H22荷瘤小鼠为模型,设置阴性组、灯台叶醇提物低中高剂量组(0.2,1.0,5.0 g/kg),阳性药环磷酰胺组,检测其抑瘤效果、碳廓清能力、耳肿胀度、血清中GR、GSH-PX、CAT、SOD和MDA含量。结果灯台叶醇提物高剂量能够明显抑制小鼠H22实体瘤生长(P<0.05),其抑瘤率为61.7%;高剂量组对小鼠耳肿胀度无明显影响,但增强其碳廓清能力,与阴性对照组比较,差异有统计学意义(P<0.05);高剂量组中血浆MDA含量水平明显降低(P<0.05),GR、GSH-PX、CAT、SOD活性水平比阴性对照组明显升高,还能够降低移植瘤细胞中活性氧ROS(P<0.05)。结论灯台叶醇提物能够增强小鼠的免疫功能;清除自由基,减轻活性氧带来的损伤,从而起到抗肿瘤作用。
Objective To study the immunoregulating and antioxidant effect of theethanol extract of Alstonia Scholaris（AS） on tumor-bearing mice.Methods H22 cells were injected subcutaneously into kunming male mice to make xenograft animal models.Tumor-bearing mice were divided into negative control group,positive control group（cyclophosphamide）,AS low,medium and high dose groups（0.2,1.0 5.0 g/kg） by intragastric administration for 16 consecutive days.The effect of AS on carbon clearance,ear swelling,the levels of serum GR,GSH-PX,CAT,SOD and MDA were measured.Results The high-dose of AS exhibited significant inhibitory effect on tumor growth（P0.05）,and the inhibition rate was 61.7%.No significant effect on mice ear swelling was noticed high dose group exhibited,But the carbon clearance was enhanced（P0.05）;Plasma MDA levels were significantly lower in the high dose group（P0.05）,The levels of GR,GSH-PX,CAT,SOD activity were significantly higher than the negative control group,and it could reduce the transplanted tumor cells reactive oxygen species ROS（P0.05）.Conclusions AS can enhance immune function and increase the activity of antioxidant enzymes,clear free radicals,thereby reduce free radical damage to the body,and finally protect the mice against the tumor.
出处
《中国热带医学》
CAS
2013年第4期414-416,共3页
China Tropical Medicine
关键词
灯台叶醇提物
H22肝癌
免疫调节
抗氧化
Alstonia scholaris（AS）
H22 liver cancer
Immunoregulating
Antioxidation
