摘要
目的:建立测定罗布麻叶饮片中金丝桃苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Phenomenex Luna C18(250mm×4.6mm,5μm),流动相为乙腈-0.015mol/L磷酸二氢钾溶液(1∶9,V/V,用氢氧化钾或磷酸调pH=5.0),流速为1.0ml/min,柱温为30℃,检测波长为260nm。结果:金丝桃苷的质量浓度在10～200μg/ml范围内与其峰面积积分值呈良好的线性关系(r=0.9998);精密度、稳定性、重复性试验的RSD均<2%;平均加样回收率为97.8%,RSD=1.5%(n=9)。结论:该方法简单、可行,结果准确、重复性好,可用于罗布麻叶饮片的质量控制。
OBJECTIVE： To establish a method for the content determination of hyperoside in Apocynum venetum decoction pieces. METHODS：RP-HPLC method was adopted. The determination was performed on Phenomenex Luna C18（250mm×4.6mm,5μm） column with mobile phase consisted of acetonitrile-0.015 mol/L potassium dihydrogen phosphate solution （ 1 ： 9, V/V, pH ad- justed to 5.0 with KOH or phosphoric acid） at the flow rate of 1.0 ml/min. The column temperature was 30 ℃ and detection wave-length was 260 nm. RESULTS： The linear range of hyperoside were 10-200 μg/ml （r=0.999 8） with average recovery of 97.8% （RSD = 1.5 %, n=9）. RSDs of precision, stability and reproducibility tests were all lower than 2 %. CONCLUSIONS：This method is simple, feasible, accurate and reproducible. It can be used for the quality control ofA. venetum decoction pieces.
出处
《中国药房》
CAS
CSCD
2013年第23期2180-2181,共2页
China Pharmacy
