摘要
目的旨在建立采用HPLC法同时测定罗布红麻和罗布白麻叶中主要黄酮类成分的方法，同时以新疆产2种罗布麻叶为实验材料，拟揭示其与其他产地罗布麻主要黄酮类成分的差异，为罗布麻质量控制提供依据。方法采用HPLC法，色谱柱为AgilentTC-C_18（150mm×4．6mm，5μm），柱温40℃，以乙腈．0．1％磷酸溶液（16：84）为流动相，体积流量为1．0mL／min，检测波长360nm，进样量10μL，测定罗布麻叶乙醇提取物中芦丁、金丝桃苷和异槲皮苷的量。结果芦丁、金丝桃苷和异槲皮苷的线性范围分别为4．96～49．60、0．96～9．60和10．24～102．40μg／mL，相关系数尺。分别为0．9992、0．9990和0．9970；加样回收率分别为98．2％、92．3％和100．1％，RSD分别为1．5％、3．2％和2．7％。结论本方法操作简单、结果准确，具有较好的重复性和稳定性，可用于同时测定罗布红麻和罗布白麻叶中3种黄酮类成分，同时，由测定结果可知，新疆产与其他产地的罗布麻叶芦丁和异槲皮苷量差异很大，新疆产罗布红麻芦丁质量分数很高，为O．55％，与异槲皮苷量相当，但金丝桃苷质量分数很低，仅O．07％，而新疆产罗布白麻的芦丁和金丝桃苷质量分数均很低。该研究将为罗布麻叶药材及其提取物质量控制提供科学依据。
Objective To establish an HPLC method for the simultaneous determination of the main flavonoids in the leaves of Apocynum venetum and Poacynum hendersonii, and to reveal the differences of flavonoids in Apocyni Veneti Herba located between Xinjiang and other areas in China. Methods The leaves of the two kinds ofApocyni Veneti Herba were extracted with 60% ethanol by reflux extraction. The separation was carried out on an Agilent TC-C18 column （150 mm × 4.6 mm, 5μm） eluted with the mobile phases of acetonitrile-0.1% phosphoric acid （16：84）. The column temperature was 40 ℃, and the flow rate was 1.0 mL/min, the detection wavelength was 360 nm, and the column temperature was 40 ℃. Results The linear ranges of rutin, hyperin, and isoquercitrin were 4.96-49.60 （r = 0.999 2）, 0.96-9.60 （r = 0.999 0） and 10.24-102.40 μg/mL （r = 0.997 0）, respectively; and the average recoveries （n = 5） were 98.2% （RSD = 1.5%）, 92.3% （RSD = 3.2%）, and 100.1% （RSD = 2.7%）, respectively. Conclusion This method is simple, rapid, accurate, and reproducible, and the results show that the flavonoids in Apocyni Veneti Herba located in Xinjiang are remarkably different from those in the other areas in China. In the leaves ofA. venetum located in Xinjiang, the lutin content is high, about 0.55%, close to the isoquercetin content, but the hypercetin content is very low, only about 0.07%, and in the leaves of P. hendersonii, the contents of both lutin and hypercetin are very small. Such study will offer the credible method for the quality control ofA. venetum and P. hendersonii.
出处
《中草药》
CAS
CSCD
北大核心
2014年第9期1326-1329,共4页
Chinese Traditional and Herbal Drugs
基金
新疆维吾尔自治区自然科学基金资助项目(2012211A021)
关键词
罗布红麻
罗布白麻
芦丁
金丝桃苷
异槲皮苷
HPLC
Apocynum venetum Linn.
Poacynum hendersonii （Hook. f.） Woodson
rutin
hyperin
isoquercitrin
HPLC
