摘要
以转TsVP基因玉米材料为研究对象,采用反复干旱法测定玉米苗期存活率和反复干旱存活率,并对株高、根冠比、叶片相对含水量、叶绿素含量、丙二醛含量、相对电导率和籽粒产量等指标进行抗旱性考察,通过方差分析、灰色关联度分析,计算抗旱隶属度。结果显示,P<0.05时,关联系数大小为株高>REC>根冠比>MDA含量>RWC>Chl含量,隶属度大小为TsVP5>TsVP6>Y478;苗期存活率受品种和干旱处理次数等因素的影响,差异显著;Chl含量为辅助指标,其他为主要指标;品种抗旱性大小为TsVP5>TsVP6>Y478。
Taking TsVP transgenic maize as material, we identified the survival rate as well as repeated drought survival rate after repeated drought stress. Some indexes had been picked for drought resistance identification, and the indexes included plant height, root shoot ratio, relative water content, chlorophyll concentration, MDA content, relative electric conductivity and the output. Throught variance analysis, gray correlation degree analysis, drought degree of membership was calculated and the resuhs displayed that the correlation coefficient was that plant height 〉 REC 〉 root shoot ratio 〉 MDA content 〉 RWC 〉 Chl content, degree of membership was TsVP5 〉 TsVP6 〉 Y478, when P value 〈 0.05. The survival rate was influenced by variety and times of treatment with significant difference, Chl content was the supplementary index while the other items were primary indexes, drought resistance among all rarities were TsVP5 〉 TsVP6 〉 Y478.
出处
《生物技术进展》
2013年第3期206-210,共5页
Current Biotechnology
基金
北京市农林科学院科技创新能力建设专项(KJCX201102003)资助
关键词
转基因玉米
抗旱性鉴定
灰色关联度分析
隶属度
genetically modified maize
drought resistance
gray correlation degree
degree of membership
