摘要
目的:研究人参皂苷Re对短波紫外线(UVC)辐射损伤人胚胎纤维芽细胞RSa的保护作用,探讨人参皂苷Re抗UVC辐射的作用机制。方法:采用不同强度的UVC辐射RSa细胞,在辐射前后给予50mg.L-1的人参皂苷Re(UVC+Re组),同时设UVC模型组和UVC+Rg1组,利用MTT法检测细胞生存率,采用流式细胞术(FCM)检测细胞周期和凋亡情况;酶生化法检测细胞内超氧化物歧化酶(SOD)的活性,集落形成分析法检测SOD抑制剂作用细胞后细胞的生存率。结果:MTT法,与UVC模型组比较,UVC+Re和UVC+Rg1组细胞生存率比较差异有统计学意义(P<0.05,P<0.01)。FCM,不同剂量(5、10和15J.m-2)的UVC辐射可引起RSa细胞早期的凋亡,UVC+Re组细胞凋亡率小于UVC模型组。细胞周期检测,UVC辐射可导致G2期细胞百分比增加(P<0.01),UVC+Re组G2期细胞百分比例明显小于UVC模型组(P<0.01)。UVC+Re组SOD活性明显高于UVC模型组(P<0.01)。结论:人参皂苷Re有较好的抗UVC效果,能够有效提高RSa细胞的生存率,减少UVC辐射引起的细胞早期凋亡,增加RSa细胞内SOD活性。
Objective To investigate the protective effects of ginsenoside Re on human embryonic fibroblast RSa cells damaged by ultraviolet C（UVC）,and to discuss the anti-UVC mechanism of ginsenoside Re.Methods The RSa cells were irradiated with different dosages of UVC,and the RSa cells were treated with 50 mg·L-1 ginsenosides before and after irradiation（UVC＋Re group）,at the same time,UVC model group and UVC＋Rg1 group were established.MTT assay was used to analyze the survival rate of cells;apoptosis and cell cycle were measured by flow cytometry（FCM） method,and the activity of superoxide dismutase（SOD） was determined by enzyme biochemical methods.The survival rate of cells after treated with SOD inhibitor was detected by colony formation assay.Results The results of MTT assay showed that the survival rate of the cells in UVC＋ Re group and UVC＋ Rg1 group were significantly increased compared with UVC model group（P0.01 or P0.05）.The FCM results showed that different dosages of UVC could induce early apoptosis of the RSa cells.The apoptotic rate of cells in UVC＋Re group was significantly decreased compared with UVC model group.The cell cycle detection results showed that the percentage of the RSa cells at G2 phase was significantly increased after UVC irradiation（P0.01）,and the percentage of the Rsa cells at G2 phase in UVC＋ Re group was significantly decreased compared with UVC model group（P0.01）,and the ratio of the RSa cells at G2 phase in UVC＋Re group was significantly decreased.Compared with UVC model group,the activity of SOD in UVC＋ Re group was incresed（P0.01）.Conclusion Ginsenoside Re has anti-UVC efficiency and can enhance the survival rate of RSa cells,and inhibit early apoptosis and increase the SOD activity.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期507-511,I0001,共6页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(200905102)
