摘要
目的:探讨银杏叶提取物(GBE)对链脲佐菌素(STZ)和盐酸异丙肾上腺素(ISO)协同诱发大鼠糖尿病心肌病(DCM)的治疗作用。方法:20只雄性Wistar大鼠随机分为正常对照组、模型组、阳性对照组和治疗组,每组5只。正常对照组大鼠不予处理,其他3组大鼠给予STZ和ISO造模,模型组大鼠给予生理盐水灌胃,阳性对照组和治疗组大鼠造膜后分别灌胃给予盐酸贝那普利和GBE,观察各组大鼠体质量、血糖水平、血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、转化生长因子β1(TGF-β1)蛋白表达水平和心肌组织形态学的变化。结果:给药1和2周时,模型组大鼠体质量、血糖水平较正常对照组降低(P<0.05),阳性对照组和治疗组大鼠体质量、血糖水平大鼠较模型组降低(P<0.05)。模型组大鼠AST、LDH和TGF-β1水平较正常对照组降低(P<0.05),阳性对照组和治疗组大鼠AST、LDH和TGF-β1水平较模型组升高(P<0.05)。正常对照组大鼠心肌无细胞肿胀;模型组大鼠心肌细胞出现界限清晰、多发散在坏死灶,坏死灶内有旺盛的成纤维细胞增生,并可见一定量的胶原纤维;治疗组大鼠心肌细胞坏死病灶面积减小,纤维化程度减轻。结论:GBE可以缓解STZ和ISO协同诱导的大鼠心肌损害。
Objective To investigate the therapeutic effect of gink gobioba extract（GBE） on diabetic cardiomyopathy（DCM） induced by streptozotocin（SZT） and isoprenaline（ISO） in rats.Methods 20 male Wistar rats were randomly divided into normal control group（n=5）,model group（n=5）,positive control group（n=5）,and treatment group（n=5）.The rats in normal control group were given nothing and the other rats were given STZ and ISO to construct DCM rat models.The rats in model group were given saline and the rats in positive control group and treatment group were given benazepril and GBE,respectively.The body weight,blood sugar level,serum AST and LDH levels,expression level of TGF-β1 protein,and morphology of myocardium of rats in various groups were observed.Results 1 and 2 weeks after treatment,the body weight and blood sugar level of the rats in model group were lower than those in normal control group（P0.05）.The body weights and blood sugar levels of the rats in positive control and treatment group were lower than those in normal control group（P0.05）.The levels of AST,LDH,and TGF-β1 in model group were higher than those in normal group（P0.05）;and the levels of AST,LDH,and TGF-β1 in positive control and treatment group were higher than those in model group（P0.05）.The myocardial cells of the rats in normal control group had no swelling.The cells in model group showed clear boundaries and more scattered necrosis,and there were lots of fibroblasts and some collagen fibers in focal necrosis.The myocardial cells of the rats in treatment group had smaller focal necrosis and lower fibrosis.Conclusion GBE can alleviate myocardial injury induced by STZ plus ISO of rats.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期503-506,I0001,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(201101067
YYZX201259)
广东省天普项目基金资助课题(2012099)
