摘要
目的:探讨奥扎格雷钠(OZG)对大鼠心肌缺血的抗氧化作用,阐明其在心肌缺血时减少心肌氧化损伤的作用。方法:健康Wistar大鼠40只随机均分为5组,即空白组、模型组、预防组、治疗组和联合硝酸甘油(GT)治疗组。预防组给予OZG治疗2周后,除空白组外其余各组均用盐酸异丙肾上腺素(ISO)制作心肌缺血模型。治疗组和联合治疗组在造模成功5 min时分别给予OZG、OZG联合GT治疗,并记录心电图。1 h后检测血清中心肌酶(AST,LDH,CK)、丙二醛(MDA)活性和超氧化物歧化酶(SOD)水平。结果:当造模成功时各组心电图ST段抬高≥0.1 mV,其中预防组ST段抬高值最小。模型组在造模成功后5 min时,抬高的ST段稍有下降,但仍≥0.1 mV;而预防组下降显著;治疗组和联合治疗组用药后的ST段与造模成功时比较,抬高的ST段均下降(P<0.05),联合治疗组最明显。与空白组比较,模型组中AST、LDH和CK三项指标均显著升高(P<0.05);预防组、治疗组、联合治疗组升高的心肌酶数值低于模型组(P<0.05)。与空白组比较,模型组造模成功时MDA升高(P<0.05),SOD下降(P<0.05)。与模型组比较,预防组和联合治疗组中升高的MDA降低,同时SOD的下降减少(P<0.05);与治疗组比较,预防组和联合治疗组中的SOD升高(P<0.05)。结论:OZG可以增强心肌细胞的抗氧化能力,降低活性自由基的损伤。
Objective To disccuss the antioxidative effects of sodium ozagrel（OZG） in myocardial ischemia in rats and illuminate its effect of decreasing oxidative damage.Methods Forty healthy Wistar rats were randomly devided into five groups：control,model,pretreatment,treatment and therapeutic alliance groups.OZG was used for 2 weeks in pretreatment group,then isoprenaline hydrochloride （ISO） was applied to make myocardial ischemia model.5 min after the establishment of model,OZG and OZG combined with glyceryl trinitrate（GT） were used in treatment group and therapeutic alliance group,respectively,ECG was recorded at the same time.1 h later,the activities of serum myocardial creatases（AST,LDH,CK）,malonaldehyde（MDA） level and superoxide dismutase（SOD） activity were detected.Results ECG：When the model was successfully set up,ST segment in every group all raised≥0.1 mV,the minimum value of raise was in pretreatment group.When the model was succeeded 5 min later,ST segment in model group slightly decreased but still ≥0.1 mV and it was significant in pretreatment group.In treatment and therapeutic alliance groups,compared with model succeeding＇ST segment,ST segment decreased significantly at 5 min later（P〈0.05）,the best obvious in therapeutic alliance group.Myocardial creatases：compared with control group,AST,LDH and CK all raised in model group respectively and all had significant difference（P〈0.05）.In pretreatment,treatment and therapeutic alliance groups,myocardial creatases were lower than those in model group（P〈0.05）.Compared with control group,when model succeeding,MDA raised（P〈0.05） and SOD decreased in model group（P〈0.05）.It also appeared in pretreatment and therapeutic alliance groups compared with model group（P〈0.05）.Compared with treatment group,SOD raised in pretreatment and therapeutic alliance groups（P〈0.05）.Conclusion OZG could enhance antioxygen capability in myocardial cells,and decrease oxidative damage by the active free radical.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期631-634,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200505199)
