摘要
目的研究食管鳞癌组织及癌细胞株中环氧合酶(COX)-2及15-羟基前列腺素脱氢酶(15-PGDH)的表达及临床意义。方法采用免疫组化(PV-9000)法检测正常食管黏膜(对照组,n=35)、食管鳞癌组织(n=35)及食管癌细胞株EC9706中COX-2和15-PGDH蛋白表达,RT-PCR法检测食管鳞癌组织及对照组15-PGDH mRNA表达。结果与对照组比较,食管鳞癌组织COX-2蛋白表达较高,15-PGDH蛋白及15-PGDH mRNA表达减低(均P<0.05)。COX-2、15-PGDH在不同浸润深度、有无淋巴结转移食管鳞癌中比较差异有统计学意义(P<0.05),在不同性别、年龄及肿瘤分化程度组织中比较差异无统计学意义。食管癌细胞株中COX-2阳性表达,15-PGDH阴性表达。结论食管鳞癌组织及癌细胞株中COX-2表达增高,15-PGDH表达减少,两者可能参与食管癌的发生、发展。
Objective To study the expression and clinical significance of cyclooxygenase-2 （COX-2） and 15-Hy- droxyprostaglandin dehydrogenase （15-PGDH） in esophageal squamous cell carcinoma and cancer cell strains. Methods The expressions of COX-2 and 15-PGDH were determined with immunohistochemical （PV-9000） method in normal esophageal mucosa （n=35）, esophageal squamous cell carcinoma （n=35）, and esophageal cell strains （EC9706）. The expression of 15-PGDH mRNA was determined with RT-PCR method in normal esophageal mucosa and esophageal squamous cell carcinoma. Results The expression of COX-2 was significantly higher in esophageal squamous cell carcinoma than that in normal tissue （P 〈 0.01）. The expression of 15-PGDH and 15-PGDH mRNA were significantly lower in esophageal squamous cell carcinoma than those in normal tissue （P 〈 0.05）. The expressions of COX-2 and 15-PGDH were significantly associated with invasive depth of carcinoma tissue and the status of lymph node metastasis （P 〈 0.05）. The expressions of COX-2 and 15-PGDH were not significantly associated with gender, age and tumor differentiation （P 〉 0.05）. A positive expression of COX-2 and a negative expression of 15-PGDH were observed in esophageal cell strains. Conclusion Compared to normal tissue, a higher expression of COX-2 and a lower expression of 15-PGDH were found in esophageal squamous cell carcinoma and cancer cell strains. Both COX-2 and 15-PGDH may play an important role in the occurrence and development of esophageal carcinoma.
出处
《天津医药》
CAS
北大核心
2013年第6期545-547,I0002,共4页
Tianjin Medical Journal
基金
天津医科大学第二医院科研基金(项目编号:Y0809)
关键词
食管肿瘤
癌
细胞系
肿瘤
环氧化酶2
羟前列腺素脱氢酶类
免疫组织化学
逆转录聚合酶链反应
esophageal neoplasms
carcinoma
cell line, tumor
cyclooxygenase 2
hydroxyprostaglandin dehydroge-nases
immunohistochemistry
reverse transcriptase polymerase chain reaction
