摘要
目的:探讨CD40和COX-2在食管鳞状细胞癌组织中的表达情况、临床病理意义及其与肿瘤血管生成的关系。方法:采用免疫组织化学方法研究79例食管癌和28例正常食管黏膜上皮组织中CD40和COX-2的表达情况,根据CD34表达计算食管癌组织平均微血管密度(MVD);分析CD40、COX-2的表达与食管癌发生部位、肿瘤大小、分化程度、MVD及淋巴结转移的关系。同时采用免疫细胞化学染色及Western Blot方法检测食管癌Eca109细胞和原代培养的正常食管上皮细胞CD40和COX-2在蛋白水平上表达的差异。结果:免疫组化结果表明,食管癌组织中CD40和COX-2的阳性表达率均明显高于正常食管鳞状上皮(54.43%vs10.71%,69.62%vs17.86%,P均<0.05)。CD40在食管癌中的表达与淋巴结转移有关,伴有淋巴结转移者CD40阳性表达率明显高于无淋巴结转移者(70.37%vs46.15%,P<0.05),但CD40表达与其他临床病理特征无明显相关。COX-2在食管癌中的阳性表达与肿瘤大小、部位、分化程度、有无淋巴结转移等均无明显相关(P>0.05)。CD40与COX-2在食管癌组织中表达明显相关(P<0.05),相关系数(Φ)为0.446。食管癌组织中MVD明显高于正常食管组织(25.02±5.52vs12.09±4.55,P<0.05)。CD40和COX-2阳性表达组食管癌组织MVD明显高于阴性表达组(26.37±6.02vs22.58±5.25,P<0.05)。体外研究结果表明,食管癌Eca109细胞CD40和COX-2表达均高于正常食管上皮细胞(P<0.05)。结论:CD40表达与食管鳞状细胞癌的发生、发展密切相关,CD40可能通过影响COX-2的表达促进食管癌组织微血管生成。
Objective： To explore the putative role of CD40 and COX-2 expression in human esophageal squamous cell carcinoma （ESCC） and to analyze their possible relationship with angiogenesis in ESCC. Meth ods： The expression of CD40 and COX-2 was detected in 79 ESCC and 28 normal esophageal epithelial tissue samples with immunohistochemical staining. The microvessel density by CD34 was determined and the clinicopathological significance of CD40 and COX-2 expression in ESCC was analyzed. In addition, the expression of CD40 and COX-2 in esophageal squamous cell carcinoma cell line Eca109 and primary cultured normal esophageal epithelial cells in vivo was comparatively studied with immunocytochemical staining and Western blot. Results： Compared with that in normal epithelial tissues, the expression of CD40 and COX-2 in ESCC was significantly higher （54.43% vs 10.71%; 69.62% vs 17.86%; respectively, P〈0.05）. The positive ex-pression rate of CD40 in ESCC cases with lymph node metastasis was significantly higher than that in those without lymph node metastasis （70.37% vs 46.15%, P〈0.05）. No correlation was found between CD40 expression and patient age, sex, tumor location, size and differentiation of tumors. No clinicopathological significance of COX-2 expression in ESCC was found. There was a positive correlation between CD40 expression and COX-2 expression in esophageal squamous cell carcinoma （P〈0.05, Φ=0.446）. The mean MVD value in ESCC was significantly higher than that in normal esophageal tissue （25.02±5.52 vs 12.09±4.55, P〈0.05）. MVD value in ESCC was closely correlated with lymph node metastasis. The mean MVD value in ESCC cases with positive CD40 and COX-2 expression was higher than that in those with negative CD40 and COX-2 expression （26.37±6.02 vs 22.58±5.25, P〈0.05）. Western blot results showed that CD40 and COX-2 expression in Ecal09 was higher than that in cultured normal esophageal epithelial cells （P〈0.05）. Conclusion： The expression of CD40 is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma. CD40 may play an important role in the angiogenesis of ESCC through promoting COX-2 expression.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第1期36-39,共4页
Chinese Journal of Clinical Oncology
基金
河北省科技攻关研究重点项目(编号:07276102D)
河北省教育厅科学研究重点项目(编号:ZH200801)
河北省自然科学基金资助(编号:C2009001098)
