摘要
目的探讨病毒性肝炎(乙肝)患者 T 细胞亚群,mIL-2R,sIL-2R,IL-6,IL-8,TNF-α与乙肝发病机制的关系.方法采用 APAAP 技术和 ELISA 法检测92例慢性乙型肝炎患者 T 细胞亚群,mlL-2R 和血清 sIL-2R,IL-6,IL-8,TNF-α水平.结果慢性乙型肝炎患者 CD_4^+细胞数较正常低(P<0.05),对照组和实验组(慢性乙肝轻度、慢性乙肝中重度、肝炎后肝硬变、重症肝炎)的 CD_4^+数值分别为:(45.6±3.6)%,(39.7±10.2)%,(40.6±11.0)%,(39.0±6.5)%,(31.2±8.9)%.CD_8^+细胞数显著上升(P<0.05或 P<0.01),对照组和实验各组依次为:(28.7±3.2)%,(35.4±9.5)%,(38.7±7.6)%,(42.2±9.4)%,以致 CD_4^+/CD_8^+比值下降;mIL-2R 显著低于正常对照组(P<0.05),在 PHA 激活后与正常接近,但均较PHA 激活前显著增高(P<0.01);PHA 激活前的 mIL-2R 对照组与实验组分别为:(15.69±4.32)%,(3.98±5.36)%,(9.37±5.48)%,(9.77±5.76)%,(9.58±5.45)%.PHA 激活后mIL-2R 对照组与实验组分别为:(69.82±5.36)%,(67.98±3.69)%,(69.11±2.19)%,(63.93±1.32)%,(66.32±5.26)%.慢性乙肝患者血清中 sIL-2R,IL-6,IL-8,TNF-α分别为:(798.9±69.0)ng/L,(2806.2±211.7)ng/L,(480.6±32.4)ng/L.正常对照组<100 ng/L,且在慢性肝炎、肝硬变活动期与稳定期之间、重型肝炎的肝坏死与恢复期之间,血清IL-6,IL-8,FNF-α三项指标的差异有显著性,均 P<0.001.结论乙肝患者存在免疫调节紊乱,而免疫异常至少有部分原因是细胞因子的作用.
AIM To investigate the expression of mIL-2R and T-cell subset,and changes of sIL-2R,IL-6,IL-8,TNF-α in patients with chronic hepatitis B. METHODS APAAP and ELISA techniques were used to detect the levels of mIL-2R and T-cell subset in peripheral blood and serum slL-2R,IL-6,IL-8 and TNF-α in 92 patients with chronic hepatitis B. RESULTS The count of CD_4~1 cell was lower whereas the count of CD_8^+ cell in patients was higher than that in normal control group (P<0.05).The count of CD_4^+ cell in control and chronic hepatitis B groups (mild chronic hepatitis,moderate to severe chronic hepatitis,post- hepatitis cirrhosis,fulminate hepatitis) were: (45.6±3.6)%,(39.7±10.2)% and (40.6±11.0)%, (39.0±6.5)% and (31.2±8.9)%.The count of CD_8^+ cell in control and chronic hepatitis B groups were: (28.7±3.2)%,(35.4±9.5)%,(38.7±7.6)% and (42.2±9.4)%.The level of mIL-2R was significantly decreased in patients and increased to normal level after PHA simulation.The expression ratio of mIL-2R before PHA simulation in control and chronic hepatitis B groups were:(15.69±4.32)%,(3.98±5.36)%,(9.37±5.48)%, (9.77±5.76) % and (9.58±5.45)%.After PHA simulation in control and chronic hepatitis B groups were: (69.82±5.36)%,(67.98±3.69)%,(69.11±2.19)%, (63.93±1.32)% and (66.32±5.26)%.However,the level of sIL-2R,IL-6,IL-8 and TNF-α were increased significantly in patients (P<0.01).The level of TNF-α,IL-6 and IL-8 in serum of moderate to severe chronic hepatitis were: (798.9±69.0)ng/L,(2806.2±211.7)ng/L,(480.6±32.4) ng/L,and <100ng/L in control group.There was significant difference of TNF-α,IL-6 and IL-8 between cirrhosis active and stable phase patients,and liver necrosis and recovery phase severe hepatitis. CONCLUSION Immunoregulation abnormality existed in patients with chronic hepatitis B,which was partly related to cellular factors.
出处
《世界华人消化杂志》
CAS
2000年第7期763-766,共4页
World Chinese Journal of Digestology
关键词
乙型肝炎
T淋巴细胞亚群
IL-2
IL-6
IL-8
hepatitis B
T-lymphocyte subsets
interleukin-2
interleukin-6
interleukin-8
receptors
