摘要
目的了解临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)的葡萄球菌盒染色体(SCCmec)基因型分布特点及耐药状况。方法多重聚合酶链反应(PCR)对MRSA进行SCCmec基因分型;普通PCR对MRSA进行4种耐药基因扩增;应用纸片扩散法测试MRSA对19种抗菌药物的敏感性。结果 SCCmec分型显示60株受试MRSA为SCCmecⅢ型菌株,4株为SCCmecⅣa型菌株,1株未分型。65株MRSA临床株对多种抗菌药物耐药,对氯霉素耐药率(4.62%)相对较低,对利奈唑胺、奎奴普丁/达福普汀、万古霉素、替考拉宁均敏感。4种耐药基因的阳性率分别为aac(6')/aph(2'')93.85%、aph(3')Ⅲ52.31%、tetM 92.31%、ant(4')9.23%。结论临床分离的MRSA SCCmec基因型以Ⅲ型为主,且具有多重耐药的特征,对氨基糖苷类、四环素类耐药基因有较高的携带率,多重PCR可以有效地用于大批量临床分离的MRSA SCCmec分型研究。
Objective To investigate the genotype distribution characteristics of staphylococcal cassette chromosome mec （SCCmec） and antimicrobial resistance of methieillin-resistant Staphylococcus aureus （MRSA）. Methods Multiplex polymerase chain reaction （PCR） was used to detect SCCmec genotype. Four antimicrobial resistant genes were amplified by traditional PCR. Antimicrobial susceptibility of 19 antibiotics was detected by disk diffusion method. Results The 60 isolates of MRSA were SCCmee Ⅲ, only 4 isolates were SCCmee Ⅳ, and 1 isolate had no genotype. All of the 65 isolates of MRSA were sensitive to linezolid, quinupristin/dalfopristin, vancomycin and teicoplanin, and were low resistant to chloramphenicol （4.62%）. The positive rates of aac （ 6′ ）/aph （ 2″ ）, aph （ 3′ ） m, terM and ant（4′） were 93.85% ,52.31% ,92.31% and 9.23%. Conclusions The main SCCmec genotype of MRSA is SCCmec Ⅲ. MRSA has multiple resistances to commonly used antibiotics. There are high percentages of aminoglycoside and tetracycline. The multiplex PCR can be applied into genotyping study of MRSA SCCmee effectively.
出处
《检验医学》
CAS
2013年第5期357-361,共5页
Laboratory Medicine
基金
山东省自然科学基金资助项目(ZR2009CL002)
山东省高校科技计划项目(J09LF67)
关键词
耐甲氧西林金黄色葡萄球菌
耐药性
葡萄球菌盒染色体
聚合酶链反应
Methicillin-resistant Staphylococcus aureus
Antimicrobial resistance
Staphylococcal cassette chromosome mec
Polymerase chain reaction
