摘要
目的了解临床分离的耐甲氧西林表皮葡萄球菌(MRSE)和耐甲氧西林溶血葡萄球菌(MRSH)中,常见耐药基因存在状况及红霉素对克林霉素的诱导耐药率。方法用PCR技术检测耐药基因,采用CLSI(NCCLS)推荐的D试验方法测定红霉素对克林霉素的诱导耐药表型。结果7株MRSE中,mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′,4″)、ermA/B/Ct、etM、vanA、vanB和qacA/B基因的阳性株数分别为7、3、1、2、3、0、0、0和2;5株MRSH中此9种基因的阳性株数分别为5、4、2、1、4、0、0、0和1;对红霉素耐药而对克林霉素敏感的5株MRSE和5株MRSH中D试验阳性株数分别为3株和1株。结论临床分离的MRSE和MRSH中存在mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′,4″)、ermA/B/C和qacA/B基因;D试验检测红霉素对克林霉素的诱导耐药性有助于临床医生正确选用MLSB类抗菌药物。
OBJECTIVE To investigate the antimicrobial-resistant genes and the percentage of inducible resistance of erythromycin to clindamycin in meticillin-resistant Staphylococcus epidermidis （MRSE） and meticillin-resistant S. haemolyticus（MRSH） isolated from clinical samples. METHODS The antimicrobial-resistant genes were analyzed by PCR. The inducible resistance of erythromycin to clindamycin were checked by D-test according to the standards of CLSI （NCCLS）. RESULTS In 7 isolates of MRSE, the positive strain numbers of genes of mecA,aac （6′）/aph（2＂）,aph（3′）-Ⅲ ,ant（4′,4＂）,ermA/B/C,tetM,vanA,vanB,and qacA/B were 7,3,1,2,3,0,0,0, and 2, respectively, and in 5 isolates of MRSH were 5,4,2,1,4,0,0,0, and 1, respectively. The positive strain numbers of inducible resistance to clindamycin（D-test positive） were 3 and 1 in 5 isolates of MRSE and 5 erythromycin resistant and clindamycin sensitive isolates of MRSH were tested by individual disk diffusion test. CONCLUSIONS At least 6 kinds of resistance genes exist including mecA, aac（6′）/aph（2＂）, aph（3′）-Ⅲ , ant（4′, 4″）, ermA/B/C, and qacA/B in MRSE and MRSH isolated from clinical samples. The inducible resistance of erythromycin to clindamycin should be checked by D-test in clinical microbiology laboratory in order to help physicians to select MLSB antimicrobial agents correctly.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2006年第10期1081-1085,共5页
Chinese Journal of Nosocomiology
基金
CLON-GEN细菌耐药基因研究专项基金资助(20031201HZ)
关键词
耐甲氧西林表皮葡萄球菌
耐甲氧西林溶血葡萄球菌
耐药基因
红霉素
克林霉素
诱导耐药
Meticillin-resistant Staphylococcus e pidermidis
Meticillin-resistant Staphylococcus haemol yticus
Antimicrobial-resistant genes
Erythromycin
Clindamycin
Inducible resistance
