摘要
为探索有效的登革热治疗方法 ,根据DEN - 2基因结构的特点 ,按照锤头状核酶的结构模型和作用模式 ,设计、合成了针对 172～ 174GUC位点的核酶基因 ;其末端分别加上SacI和SalI接头 ,定向插入质粒pGEM - 3zf (+ )的SacI和SalI位点之间 ;其引导序列中的PvuII位点 ,提供了快速简便的核酶克隆的鉴定方法。PvuII酶切鉴定的核酶基因克隆经序列分析 ,结果与预期完全一致。证明得到了特异切割DEN - 2基因的核酶基因克隆 ,为进一步核酶抗DEN -
To construct a specific hammerhead ribozyme possessing catalytic activity that cleaves the dengue virus gene, a DNA sequence encoding the ribozyme gene was synthesized, and then incorporated into the Sac I and Sal I site of vector pGEM3Zf(+). The recombinant plasmids were screened out by digestion with Pvu II. Sequence analysis showed that a clone to express a hammerhead ribozyme targeting the 172~174 GUC triplet of DEN-2 has been obtained.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第5期11-12,19,共3页
Chinese Journal of Zoonoses
基金
广东省医学科研课题!A1998493
