摘要
目的对1株海洋来源的真菌小红酵母Rhodotorula minuta.次级代谢产物进行活性化学成分的分离研究。方法通过抗氧化(DPPH法)和抗肿瘤(MTT法)双模型活性追踪筛选分离菌株发酵液正丁醇萃取部分的活性化学成分,并利用流式细胞仪技术对抗肿瘤机制作初步探究。结果从中得到了1个较高活性环二肽类化合物,利用波谱分析及与文献数据对照等方法鉴定为环亮-异亮氨酸Cyclo(Leu-Ile),为首次从海洋小红酵母菌中分得。在浓度为10μmol.mL-1时,环亮-异亮氨酸对DPPH自由基的清除率率达73.76%;对HepG2、PC-12、U937肿瘤细胞的IC50值分别为0.538,0.635和0.437μmol.mL-1;流式细胞仪检测表明,环二肽能有效诱导U937细胞凋亡。结论该环二肽在体外具有较好的清除DPPH自由基活性,对HepG2、PC12、U937肿瘤细胞具有较好的细胞毒活性,同时也表明海洋真菌是活性天然产物的重要来源。
Objective To isolate and study active components from the secondary metabolites of a marine fungus Rhodotorula minuta. Methods The constituents from the n-BuOH extracts of the fermentation broth of R. rninuta were bioactivity-guided and separated by DPPH and MTT methods, simultaneous- ly. And the mechanism of its anti-tumor activity was preliminarily studied by Flow Cytometer （FCM） in this article. Results One active compound was separated from the broth and identified as Cyclo （Leu- Ile） by spectrum analysis and comparing with the data from reference. It＇s obtained from marine fungus R. minuta for the first time. The DPPH scavenging ratio of Cyclo （Leu-Ile） was more than 73.76% when its concentration was 10μmol · m1-1 while its IC50 against HepG2, PC-12 and U937 were 0. 538, 0. 635 and 0. 437 μmol · ml 1, respectively. The study of FCM showed that Cyclo （Leu-Ile） can induce the U937 cells apoptosis effectively. Conclusions The Cyclo （Leu-Ile） showed satisfactory DPPH scav- enging and cytotoxicity against HepG2, PC-12 and U937 cells in vitro and also indicated that marine fungus is a significant source of natural bioactive products.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2013年第3期28-32,共5页
Chinese Journal of Marine Drugs
基金
国家自然科学基金项目(30973681)资助
关键词
海洋小红酵母
活性追踪
流式细胞仪
环二肽
Rhodotorula minuta
activityguide
flow cytometer
cyclic dipeptide
