摘要
目的噁唑烷酮类抗菌药是一类新型化学令合成抗菌药，具有抑制多重耐药革兰阳性菌的作用，本文建立高效液相色谱法测定新型噁唑烷酮类药物YC-12的含量及检查有关物质。方法采用Diamonsil C18柱（250mm×4．6mm，5μm），磷酸盐缓冲溶液（1000mL水＋2g磷酸二氢钾＋3．5mL三乙胺＋3mL磷酸）-乙腈-甲醇（42：30：28）为流动相；流速为1mL／min：检测波长254nm，柱温为25℃。结果主峰能与相邻杂质峰达基线分离，YC-12的浓度在10～200μg／mL范围内线性关系良好，相关系数为0．9994，最低检测限为0．5ng。结论该法简便、准确，灵敏度高，专属性强，可以用于YC-12的含量及有关物质的测定。
Objective Totally-synthetic oxazolidinones are a new class of antibacterial agents for the treatment of the infections caused by gram-positive bacteria including the multidrug-resistant pathogens. To develop an HPLC method for determination of new oxazolidinone derivatives YC-12 and its related substances. Methods The chromatographic separation of YC-12 was performed on a Diamonsil C18 column with a mobile phase consisting of phosphate buffered solution（1000mL H2O with 2.0g potassium dihydrogen phosphate, 3.5mL triethylamine and 3mL Phosphoric Acid）- acetonitrile-methanol（42：30：28, v/v/v） with a flow rate of 1.0mL/min. The detection was monitored at 254 nm, and the column temperature was maintained at 25℃. Results YC-12 was completely separated from its related substances. The calibration curve of YC-12 was linear in the range of 10-200μg/mL with the correlation coefficient of 0.9994. The limit of detection was 0.5ng. Conclusion The method is simple, rapid, sensitive and specific, and can be used for determination of YC-12 and its related substances.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2011年第6期445-448,共4页
Chinese Journal of Antibiotics
基金
国家高技术研究发展计划(863计划)重大科技专项。基金号2004AA2Z3201
